[1]王晶雪 王晓庆 陆叶**.盆腔器官脱垂患者子宫骶韧带线粒体DNA4977缺失及其生物学意义[J].中国微创外科杂志,2019,01(3):256-266.
　Wang Jingxue,Wang Xiaoqing,Lu Ye..Mitochondrial DNA4977 Deletion in Uterosacral Ligaments in Patients With Pelvic Organ Prolapse and Its Biological Significance[J].Chinese Journal of Minimally Invasive Surgery,2019,01(3):256-266.
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盆腔器官脱垂患者子宫骶韧带线粒体DNA4977缺失及其生物学意义()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
01
期数:
2019年3期
页码:
256-266
栏目:
基础研究
出版日期:
2019-03-25

文章信息/Info

Title:
Mitochondrial DNA4977 Deletion in Uterosacral Ligaments in Patients With Pelvic Organ Prolapse and Its Biological Significance
作者:
王晶雪 王晓庆 陆叶**
（北京大学第一医院妇产科，北京100034）
Author(s):
Wang Jingxue;  Wang Xiaoqing;  Lu Ye.
Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, China
关键词:
盆腔器官脱垂; 子宫骶韧带; 线粒体DNA4977缺失
Keywords:
Pelvic organ prolapse; Uterosacral ligament; Mitochondrial DNA4977 deletion
文献标志码:
A
摘要:
目的通过研究线粒体DNA4977缺失在盆腔器官脱垂（pelvic organ prolapse,POP）患者子宫骶韧带中的变化，初步探讨POP可能的发生机制。方法取2016年9月~2017年4月我科因POP Ⅱ~Ⅳ期切除子宫26例为研究组，同期因良性疾病切除子宫的非POP患者21例为对照组，取子宫骶韧带组织，提取其中的线粒体DNA，应用荧光定量PCR方法检测线粒体DNA和DNA4977缺失的相对含量，应用琼脂糖凝胶电泳方法对PCR产物进行比对分析。结果 ①研究组子宫骶韧带组织中线粒体DNA4977缺失明显高于对照组，差异有统计学意义（Z=-2.086,P=0.037）；而2组总线粒体DNA含量无明显差异（P=0.447）。②研究组线粒体DNA4977缺失与年龄、孕次、产次、BMI、初产年龄、绝经年龄均无明显相关性（P>0.05）;对照组患者线粒体DNA4977缺失与年龄有明显相关性，随着年龄的增加，线粒体DNA4977缺失增多（r=0.465,P=0.034），而与孕次、产次、BMI、初产年龄、绝经年龄无明显相关性（P>0.05）。结论子宫骶韧带线粒体DNA4977缺失在POP患者中显著增加，可能是导致POP的原因之一。
Abstract:
ObjectiveTo explore the pathogenic mechanism of pelvic organ prolapse (POP) through the study of mitochondrial DNA4977 (mtDNA4977) deletion in uterosacral ligaments (UL).MethodsTwentysix patients with POP stage Ⅱ-Ⅳ and 21 control patients with benign gynecological disease who underwent hysterectomy at our hospital between September 2016 and April 2017 were included. The mitochondrial DNA (mtDNA) was isolated from UL tissues and the relative quantitative level of mitochondrial DNA and DNA4977 deletion was assessed by fluorescent quantitative PCR and agarose gel electrophoresis between two groups to explore its biological significance.ResultsThe mtDNA4977 deletion rate in UL was significantly higher in the POP group as compared to the control group (Z=-2.086, P=0.037); while no significant difference was found in the total amount of mtDNA (P=0.447). In addition, no obvious correlations between mtDNA4977 deletion and age, pregnancy times, delivery times, BMI, primiparity age, and menopause age were observed in the POP group (P>0.05). In the control group, the mtDNA4977 deletion was positively correlated with age (r=0.465, P=0.034), mtDNA4977 deletion increased with age,while no significant correlations with pregnancy times, delivery times, BMI, primiparity age and menopause age were found (P>0.05).ConclusionThe mtDNA4977 deletion is significantly increased in POP patients, which might be one of the causes of POP.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（81401185）**通讯作者，Email：songqinlu@sina.com
更新日期/Last Update: 2019-06-04