[1]刘长旭 贺余 刘昶**.荧光腹腔镜下小鼠胃癌可视化成像的实验研究[J].中国微创外科杂志,2020,01(4):349-353.
　Liu Changxu,He Yu,Liu Chang..Experimental Study on Fluorescent Laparoscopic Visualization of Gastric Cancer in Mice[J].Chinese Journal of Minimally Invasive Surgery,2020,01(4):349-353.
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荧光腹腔镜下小鼠胃癌可视化成像的实验研究()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
01
期数:
2020年4期
页码:
349-353
栏目:
实验研究
出版日期:
2020-04-25

文章信息/Info

Title:
Experimental Study on Fluorescent Laparoscopic Visualization of Gastric Cancer in Mice
作者:
刘长旭 贺余 刘昶**
(哈尔滨医科大学附属第四医院普外科，哈尔滨150001)
Author(s):
Liu Changxu;  He Yu;  Liu Chang.
Department of General Surgery, Fourth Affiliated Hospital of Harbin Medical University, Harbin 150001, China
关键词:
量子点; 胃癌; 荧光腹腔镜; 裸鼠
Keywords:
Quantum dots; Gastric cancer; Fluorescent laparoscopy; Nude mice
文献标志码:
A
摘要:
目的探讨荧光腹腔镜下小鼠胃癌可视化成像在鉴别肿瘤和正常组织中的价值。方法共20只3~4周龄，体重20 g裸鼠。2只裸鼠右后肢皮下注射0.2 ml高表达HER2基因的NCIN87胃癌细胞悬液用于观察背部成瘤情况，1周后当瘤体直径达1 cm左右进行下一步实验观察。将0.2 ml高表达HER2基因的NCIN87胃癌细胞悬液注射于12只裸鼠胃黏膜下，建立裸鼠胃癌模型。将量子点与HER2的单抗结合制作量子点纳米荧光探针。将12只胃癌模型裸鼠分为A、B组，每组6只，A组裸鼠尾静脉注射量子点纳米荧光探针100 μl与裸鼠胃癌模型进行体内结合，B组裸鼠尾静脉先注射HER2单克隆抗体再注射量子点纳米荧光探针100 μl，C组6只正常生长的裸鼠尾静脉直接注射量子点纳米荧光探针100 μl。最后利用改装后的荧光腹腔镜分别以15、30 min和1、2、4、8、12、24、30 h时间点观察不同时期裸鼠体内胃癌组织及其他部位的荧光显像，标本送检。 结果裸鼠种瘤成功，胃癌组织在荧光腹腔镜下完成可视化成像，荧光探针结合后稳定性良好，荧光强度在1 h后达到峰值，30 h后荧光消失。发光组织病理切片证实为胃癌组织，免疫组化证实该组织高表达HER2蛋白。结论量子点纳米荧光探针性质稳定，荧光强度1 h达到峰值，然后逐渐衰减，荧光腹腔镜下可清晰看到高表达HER2的NCIN87胃恶性肿瘤的边界和周围组织及淋巴结的转移情况。
Abstract:
ObjectiveTo investigate the value of fluorescent laparoscopy visual imaging of gastric cancer to differentiate tumor from normal tissue in mice.MethodsA total of 20 nude mice aged 3-4 weeks and weighing 20 g were used. Two nude mice were injected subcutaneously with high expression of HER2 gene of NCIN87 gastric cancer cell fluid 0.2 ml in the right lower extremity to observe the tumorigenesis of the back. One week later, when the tumor diameter reached about 1 cm, the next experimental observation was carried out. The gastric cancer model of nude mice was established by injecting 0.2 ml NCIN87 gastric cancer cell solution with high expression of HER2 gene into gastric mucosa of 12 nude mice. The quantum dot (QD) was combined with the monoclonal antibody of HER2 to make the quantum dot nano fluorescence probe. The gastric cancer models of 12 nude mice were divided into two groups: group A and group B,6 mice in each group. In the group A, 100 μl QD nanofluorescent probe was injected into the tail vein of nude mice, and in the group B, 100 μl HER2 monoclonal antibody (0.5 μmol/L) was injected into the tail vein of nude mice, and then 100 μl QD nanofluorescent probe was injected. In the group C, six normal nude mice were injected with 100 μl QD nanofluorescence probe directly into the tail vein. Finally, the modified fluorescence laparoscopy was used to observe the fluorescence imaging of gastric cancer tissues and other parts in nude mice at 15 min, 30 min, 1 h, 2 h, 4 h, 8 h, 12 h, 24 h and 30 h respectively. Finally, the samples were sent for examination.ResultsSuccessful tumor growth was achieved in nude mice. The visual imaging of gastric cancer tissue under fluorescent laparoscopy was completed, with good stability. The fluorescence intensity reached the peak after 1 h, and the fluorescence disappeared after 30 h. The pathological section of luminescent tissue proved to be gastric cancer tissue, and immunohistochemistry confirmed the high expression of HER2 protein in this tissue.ConclusionsThe properties of the quantum dots nanofluorescence probe are stable. The fluorescence intensity reaches the peak after 1 h, and then attenuates gradually. The fluorescent laparoscopy can clearly see the boundary of high expression of HER2 gene of NCIN87 gastric cancer and the metastasis of the surrounding tissue and lymph nodes.

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备注/Memo

备注/Memo:
基金项目：哈尔滨医科大学附属第四医院特别资助项目（HYDSYTB201911）**通讯作者，Email: changliu72@163.com
更新日期/Last Update: 2020-07-14