[1]欧阳伟炜,解用江,高福平,等.热籽感应加温体内外升温情况及其对荷瘤大鼠免疫功能的影响[J].中国微创外科杂志,2009,09(6):491-494.
　Ouyang Weiwei,Xie Yongjiang,Gao Fuping*,et al.Thermoseed Inductive Heating in vivo and in vitro and Its Impact on Immune Function of Tumor Bearing Rats[J].Chinese Journal of Minimally Invasive Surgery,2009,09(6):491-494.
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热籽感应加温体内外升温情况及其对荷瘤大鼠免疫功能的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
09
期数:
2009年6期
页码:
491-494
栏目:
实验研究
出版日期:
2009-08-01

文章信息/Info

Title:
Thermoseed Inductive Heating in vivo and in vitro and Its Impact on Immune Function of Tumor Bearing Rats
作者:
欧阳伟炜; 解用江; 高福平; 唐劲天**
Author(s):
Ouyang Weiwei;  Xie Yongjiang;  Gao Fuping*;  et al.
*Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
关键词:
热籽; 热疗; 交变磁场; 感应加温; 肿瘤; 免疫功能
Keywords:
Thermoseed; Hyperthermal; Alternating magnetic field; Inductive heating; Tumor; Immune function
分类号:
R-332
文献标志码:
A
摘要:
目的评估热籽在交变磁场中体外升温及其对荷瘤大鼠加温治疗效果和对免疫功能的影响。方法监测居里点分别为57 ℃及70 ℃热籽体外升温情况。40只接种Walker256肿瘤细胞的Wistar大鼠，随机分为C组（空白对照组）10只，M组（磁场对照组）5只，T组（热籽对照组）5只，加温治疗H1组10只、H2组10只。H1组：瘤内植入2颗居里点57 ℃热籽，持续30 min；H2组：2颗居里点70 ℃热籽，持续6 min。加温治疗后9天各组均处死5只大鼠测其肿瘤体积及质量，剩余H1、H2组及C组大鼠检测外周血T淋巴细胞亚群。结果热籽体外升温可达到其居里点温度。热疗后肿瘤体积中位数H2组0.50 cm3（0.00~26.54）明显小于M组36.18 cm3（0.96~39.90）（Z=2.21，P=0.032），肿瘤质量中位数H1组0.96 g（000~21.18）、H2组0.41 g（0.00~23.40）明显小于M组31.45 g（1.09~36.09）（二者均为Z=2.21，P=0.032）。外周血T淋巴细胞亚群CD4+ H1组（22.39±5.27）%、H2组（24.76±5.19）%明显高于C组（12.07±4.45）%（P=0.01,0.003），CD8+ H2组（19.58±4.63）%明显高于C组（12.72±3.96）%（P=0.04）。结论热籽在交变磁场中能达到适宜的温度，其感应加温治疗对荷瘤大鼠肿瘤生长有一定的抑制作用，能提高免疫功能。
Abstract:
ObjectiveTo evaluate the heating ability of thermoseeds in alternating magnetic field and its therapeutic effects on tumor bearing rats with simultaneously detected immune function. MethodsTo monitor the temperature increase of thermoseeds in vitro, of which the Curie point was 57 ℃ or 70 ℃. Forty Wistar rats implanted with Walker256 cells were randomly divided into five groups: C group (control group, 10 rats), M group (magnetic control group, 5 rats), T group (thermoseeds control group, 5 rats) and two heating treatment groups (H1 group, 10 rats and H2 group, 10 rats). In the H1 group, two thermoseeds with a Curie point at 57 ℃ were implanted into the tumor tissues in each rat with a heating time of 30 minutes, while in the H2 group, two thermoseeds with a Curie point at 70 ℃ were used with a heating time of 6 minutes. Five rats from each group were killed 9 days after the heating therapy to evaluate the volume and weight of the tumor tissues. The peripheral blood Tcells were counted in the rest rats in the H1, H2, and C groups. ResultsMagnetic inductive heating of the thermoseeds in vitro can reach the Curie point. The median tumor volume in the H2 group was 0.50 cm3 (0.00-26.54), which was significantly lower than that in the M group (36.18 cm3, 096-39.90, Z=2.21, P=0.032). And the weight of the tumors in the H1 and H2 groups was significantly lower than that in the M group ［0.96 g (0.00-21.18) in H1 and 0.41 g (0.00-23.40) in H2 vs 31.45 g (1.09-36.09) in M group, Z=2.21 and P=0.032 for both］. In the peripheral blood, the percentage of CD4+ Tcells was (22.39±5.27)% in H1 group, and (24.76±519)% in H2 group, which were significantly higher than that in C group ［(12.07±4.45)%, P=0.01 and 0.003 respectively］; and the percentage of CD8+ Tcells in the H2 group was significantly higher than that in the C group ［(19.58±4.63)% vs (12.72±396)%, P=0.04］.ConclusionsThermoseed had a good heating ability in alternating magnetic field and its inductive heating can inhibit tumor growth in Wistar rats and improve the immune function of the rats.

参考文献/References:

[1]夏启胜，刘轩，李红艳，等.热籽感应加温治疗肿瘤的实验与临床研究进展.中华物理医学与康复杂志,2005,27：380-382.
[2]夏启胜，刘轩，徐波，等．热籽感应加温对荷瘤小鼠治疗效果的实验研究.中国微创外科杂志,2007,7：1031-1034.
[3]Steeves RA，Tompkins DT，Nash RN，et al．Thermoradiotherapy of intraocular tumors in an animal model：concurrent vs sequential brachytherapy and ferromagnetic hyperthermia．Int J Radiat Oncol Biol Phys，1995,33：659-662．
[4]Deger S，Boehmer D，Turk I，et al．Interstitial hyperthermia using selfregulating thermoseeds combined with conformal radiation therapy．Eur Urol，2002,42：147-153．
[5]Kobayashi T，Kida Y，Tanaka T，et al．Interstitial hyperthermia of malignant brain tumors by implant heating system：clinical experience.J Neuro Oncol，1991,10：153-163．
[6]Stea B，Kittelson J，Cassady JR，et al.Treatment of malignant gliomas with interstitial irradiation and hyperthermia．Int J Radiat Oncol Biol Phys，1992，24：657-667.
[7]Tucker RD，Platz CE，Huidobro C，et al.Interstitial thermal therapy in patients with localized prostate cancer：histologic analysis.Urology，2002，60：166-169．
[8]Berthusen AH，Tucker RD，Park JB．A fieldfocusing device to increase power output of ThermoRod trade mark implants for thermal ablation of tissue．J Biomed Mater Res，2002，63：650-656．
[9]Tucker RD．Use of interstitial temperature selfregulating thermal rods in the treatment of prostate cancer．J Endourol，2003，17：601-607.
[10]Deger S,Taymoorian K,Boehmer D,et al.Thermoradiotherapy using interstitial selfregulating thermoseeds:an intermediate analysis of a phase Ⅱ trial.Eur Urol,2004,45：574-580.
[11]Tucker RD,Huidobro C,Larson T,et al.Ablation of stage T1/T2 prostate cancer with permanent interstitial temperature selfregulating rods.J Endourol,2005,19:865-867.
[12]Deger S,Schink T,Boehmer D,et al.Match pair analysis HDR brachytherapy vs. thermoradiotherapy using interstitial thermoseeds.Eur Urol Suppl,2006,5:131.
[13]Akiyama S,Kawasaki S,Kodera Y,et al.A new method of thermochemotherapy using a stent for patients with esophageal cancer.Surg Today,2006,36:19-24.
[14]于金明，王仁本，王蔚林.肿瘤热疗的热剂量学研究进展与评价.中华肿瘤杂志，2004，26：257-259.
[15]耿跃春，王想想，胡焰,等．铁磁热籽诱导加热治疗肝癌的实验研究.中华理疗杂志,1997,20：204-206．
[16]杜春艳，刘继光，唐劲天.加温治疗肿瘤的温度与机体免疫关系.中国微创外科杂志,2007,7:1051-1053.
[17]Calderwood SK,Theriault JR,Gong JL.How is the immune response affected by hyperthermia and heat shock proteins？Int J Hyperthermia,2005,21:713-716.
[18]吴昱,姜南艳,程庆书,等.射频治疗肺癌患者免疫淋巴细胞检测.第四军医大学学报,2004,25（7）:671-672.
[19]秦颂兵，周菊英，俞志英，等.肿瘤患者局部热疗后机体免疫功能变化的初步探讨.苏州大学学报（医学版）,2006,26：1034-1035.

备注/Memo

备注/Memo:
基金项目：国家自然科学基金（10775085）；国家自然科学基金（30571779）；北京市科委（Z07000200540704）；清华大学裕元基金**通讯作者作者单位：410008中南大学湘雅医院肿瘤科（欧阳伟炜）；163001黑龙江省大庆油田总医院口腔科（解用江）；100084清华大学工程物理系医学物理与工程研究所（高福平、唐劲天）
更新日期/Last Update: 2014-01-08