[1]江薇,赵凌云,盛军,等.动脉栓塞热疗用羰基铁粉磁热效应和细胞毒性研究[J].中国微创外科杂志,2009,09(6):487-490.
　Jiang Wei,Zhao Lingyun*,Sheng Jun,et al.Magnetoheating Effect and Cytotoxicity of Carbonyl Iron Powder in Arterial Embolization Hyperthermia[J].Chinese Journal of Minimally Invasive Surgery,2009,09(6):487-490.
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动脉栓塞热疗用羰基铁粉磁热效应和细胞毒性研究()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
09
期数:
2009年6期
页码:
487-490
栏目:
实验研究
出版日期:
2009-08-01

文章信息/Info

Title:
Magnetoheating Effect and Cytotoxicity of Carbonyl Iron Powder in Arterial Embolization Hyperthermia
作者:
江薇; 赵凌云; 盛军; 唐劲天**
Author(s):
Jiang Wei;  Zhao Lingyun*;  Sheng Jun;  et al.
*Institute of Medical Physics and Engineering, Department of Engineering Physics, Tsinghua University, Beijing 100084, China
关键词:
羰基铁粉; 交变磁场; 热疗; 细胞毒性; L929细胞
Keywords:
Carbonyl iron powder; Alternating magnetic field; Hyperthermia; Cytotoxicity; L929 cells
分类号:
R-332
文献标志码:
A
摘要:
目的探讨将羰基铁粉应用于磁感应动脉栓塞加温治疗肿瘤，测定其在交变磁场下升温状况和细胞毒性，初步评价其磁热性和安全性。方法分别改变混悬液浓度（10 mg/ml羰基铁粉100%浸提液、10 mg/ml羰基铁粉50%浸提液、64 g/L苯酚溶液）和磁场电流强度（49.9、79.9、110.2 Oe），测定因浓度和磁场电流强度的变化对羰基铁粉升温的影响。将羰基铁粉浸提液对小鼠L929成纤维细胞培养2、4、7 d，显微镜下观察细胞形态变化，MTT法检测计算细胞相对增殖度，用6级毒性分类法评级。结果羰基铁粉-碘化油混悬液的温度随浓度的升高、磁场强度的增加而升高。浓度为60 mg/ml的羰基铁粉-碘化油混悬液在110.2 Oe下可以达到理想的治疗温度。细胞毒性检测，小鼠成纤维细胞L929在10 mg/ml羰基铁粉100%浸提液、10 mg/ml羰基铁粉50%浸提液培养期（培养2、4、7 d）细胞贴壁生长，形态良好，细胞毒级为0~1级。结论羰基铁粉在交变磁场下有较好的升温效果，无细胞毒性。
Abstract:
ObjectiveTo evaluate the magnetoheating effect and cytotoxicity of the carbonyl iron powder as a feasibility for arterial embolization hyperthermia. MethodsDifferent doses of carbonyl iron powder suspensions were prepared in vitro (10 mg/ml, 100%; 10 mg/ml, 50%; or 64 g/L phenol solutions), and heated for 20 minutes in an alternating magnetic field (49.9, 799, and 110.2 Oe). The influences of the doses of suspensions and currency of magnetic field on the heating effect were observed.Meanwhile, mouse fibroblast L929 cells were cultured with the suspensions for 2, 4, or 7 days. The morphology and relative growth rate (RGR) of the cells were determined by microscopy and MTT assay. The cytotoxicity of the suspensions was then classified. ResultsThe heating ability of the carbonyl iron powder increased with the suspension concentration and the strength of the magnetic field. A optimal therapeutic temperature was achieved at 110.2 Oe with 60 mg/ml carbonyl iron powder suspension. The L929 cells showed normal morphology after been treated by the carbonyl iron powder (10 mg/ml 100% solution and 50% solution) for 2, 4 and 7 days with the 0-1 degree cytotoxicity.ConclusionThe carbonyl iron powder has good heating effect under the alternating magnetic field, and is compatible with the tested cells.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金（10775085）；国家自然科学基金（30571779）；北京市科委（Z07000200540704）；清华大学裕元基金**通讯作者作者单位：100102北京中医药大学中药学院微生物与生化药学系（江薇）；100084清华大学工程物理系医学物理与工程研究所粒子技术与辐射成像教育部重点实验室（赵凌云、唐劲天）；130062长春生物制品研究所（盛军）
更新日期/Last Update: 2014-01-08