[1]石彦,余佩武*,雷晓,等.不同压力CO2气腹对人胃癌MKN45细胞生长的影响[J].中国微创外科杂志,2008,08(6):548-551.
　Shi Yan,Yu Peiwu,Lei Xiao,et al.Effect of CO2 Pneumoperitoneum on Proliferation of Gastric Cancer Cell MKN45[J].Chinese Journal of Minimally Invasive Surgery,2008,08(6):548-551.
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不同压力CO2气腹对人胃癌MKN45细胞生长的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
08
期数:
2008年6期
页码:
548-551
栏目:
基础研究
出版日期:
2008-10-31

文章信息/Info

Title:
Effect of CO2 Pneumoperitoneum on Proliferation of Gastric Cancer Cell MKN45
作者:
石彦; 余佩武*; 雷晓; 钱锋; 赵永亮; 唐波; 罗华星
第三军医大学西南医院普通外科、微创胃肠外科中心，重庆400038
Author(s):
Shi Yan; Yu Peiwu;  Lei Xiao;  et al.
Department of General Surgery and Center of Microinvasive Gastrointestinal Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
CO2气腹; 胃癌细胞; 生长增殖
Keywords:
Carbon dioxide pneumoperitoneum;  Gastric cancer cells;  Proliferation
分类号:
R735.2
文献标志码:
A
摘要:
目的研究体外模拟不同压力CO2气腹环境对人胃癌MKN45细胞生长增殖能力的影响。方法实验组于体外建立模拟CO2气腹环境，全自动气腹机维持气腹压力分别为9、12、15 mm Hg，作用时间均为4 h，对照组直接放入37℃、5% CO2孵箱中培养。处理后第0、0.5、1、1.5、2、2.5、3 h，用pH计检测细胞培养液pH值变化；第1、2、3、4、5、6、7天，MTT法检测细胞增殖情况。结果15 mm Hg CO2气腹处理后胃癌细胞增殖较对照组下降显著（P＜0.05），但9、12 mm Hg组与对照组差异无统计学意义（P＞0.05）。CO2气腹处理后细胞培养液pH值较对照组下降非常显著（P＜0.01），但在恢复正常培养后最长3 h即恢复正常。胃癌细胞增殖在第1天与不同压力CO2气腹处理后细胞培养液的即时pH值有显著相关性（P＜0.05），而第2、3、4、5、6、7天均无相关性（P＞0.05）。结论模拟9、12 mm Hg CO2气腹对胃癌MKN45细胞增殖没有显著影响，模拟15 mm Hg CO2气腹对胃癌MKN45细胞增殖有显著的抑制作用。
Abstract:
ObjectiveTo study the effect of CO2 pneumoperitoneum with different pressures on the proliferation of human gastric cancer cells MKN45.MethodsThe MKN45 cells were divided into experiment and control groups. In the experiment group, the MKN45 cells were exposed to CO2 with different pressures (9, 12, or 15 mm Hg) for 4 hours. The cells in the control group were cultured at 37℃ with 5% CO2. After being treated for 0, 0.5, 1, 1.5, 2, 2.5, and 3 hours, the pH of culture media was measured. MTT assay was employed to detect the proliferation of the cells 1, 2, 3, 4, 5, 6, and 7 days after the treatment.Results Compared with the control, the proliferation of the MKN45 cells was not affected by CO2 at a pressure of 9 or 12 mm Hg (P＞0.05), but were significantly inhibited after being treated with 15 mm Hg CO2 (P＜0.05). The pH value of the experiment group was significantly lower than that in the control (P＜0.01). After being cultured in a normal environment for at most 3 hours, the pH of the experiment group increased to a normal range. The proliferation of the cells was significantly related to the pH value of culture media determined after being exposed to CO2 pneumoperitoneum with different pressures (P＜0.05) at the first day. However, no such relationship was observed from days 2 to 7 (P＞0.05).ConclusionIn vitro, the proliferation can not be influenced by CO2 with a pressure of 9 or 12 mm Hg, but can be significantly inhibited by CO2 with a pressure of 15 mm Hg.

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备注/Memo

备注/Memo:
基金项目:全军“十一五”计划课题资助项目（06MB240）；重庆市医学重点学科建设资助项目,*通讯作者
更新日期/Last Update: 2013-10-22