[1]汪毅①,任国胜②,石远,等.透明质酸酶及基质金属蛋白酶-9在人乳腺癌侵袭与转移中的意义[J].中国微创外科杂志,2006,06(9):675-677.
　Wang Yi,Ren Guosheng*,Shi Yuan*,et al.Expression of hyaluronidase and matrixm etaloproteinase-9in the invasion and m etastasis of human breast cancer[J].Chinese Journal of Minimally Invasive Surgery,2006,06(9):675-677.
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透明质酸酶及基质金属蛋白酶-9在人乳腺癌侵袭与转移中的意义()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
06
期数:
2006年9期
页码:
675-677
栏目:
基础研究
出版日期:
2006-09-20

文章信息/Info

Title:
Expression of hyaluronidase and matrixm etaloproteinase-9in the invasion and m etastasis of human breast cancer
作者:
汪毅①; 任国胜②; 石远; 王小毅; 冉亮　
中国医学科学院中国协和医科大学肿瘤医院腹外科,北京,100021
Author(s):
Wang Yi; Ren Guosheng*;  Shi Yuan*;  et al.*
Department of General Surgery, First Affiliated Hospital of Chongqing Medical University,Chongqing 400016, China
关键词:
乳腺癌; 透明质酸酶; 基质金属蛋白酶-9; 侵袭与转移
Keywords:
Breast cancer; 　Hyaluronidase; 　Matrixmetaloproteinase-9; 　Invasion andmetastasis
分类号:
R737.9;R446.6
文献标志码:
A
摘要:
目的 研究透明质酸酶(Haase)与基质金属蛋白酶-9(MMP-9)在人乳腺癌侵袭与转移中的意义.方法 应用组织匀浆、酶连吸附测定法及免疫组化等方法测定Haase和MMP-9 在人乳腺癌中的表达和其病理特征比较.结果 ①乳腺癌组织学分级Ⅰ、Ⅱ、Ⅲ级组Haase的分别为4.89±2.55、8.03±2.66、12.00±3.96 mU/g(n=10,20,3),Ⅲ级组＞Ⅱ级组＞Ⅰ级组 (P＜0.05).乳腺癌有腋淋巴结转移组Haase表达(8.27±3.26 mU/g,n＝23)明显高于无腋淋巴结转移组(5.53±2.82 mU/g,n＝10)(t＝-2.305,P=0.028).②MMP-9阳性率在淋巴结转移组为(78.3%,18/23),明显高于无淋巴结转移组(30.0%,3/10)(P=0.016).③MMP-9阳性组(n=21) Haase( 8.57±3.12) mU/g明显高于阴性组(n=12)(5.47±2.85) mU/g(t=2.830,P =0.008).结论 Haase和MMP-9一样,与人乳腺癌侵袭与转移有关.
Abstract:
Objective　To study the expression of hyaluronidase (HAase) and matrixmetalloproteinase-9 (MMP-9) in the invasion and metastasis of human breast cancer.　M ethods　The expression ofHAase and MMP-9 in human breast cancer was detected using tissue homogenate preparing, enzyme-linked immunoadsorbent assay (ELSA), and immunohistochemistry examination. Results　①The levels ofHAasewere 4. 89±2. 55, 8. 03±2. 66, and 12. 00±3. 96 mU/g in gradeⅠ,Ⅱ, andⅢofbreastcancer, respectively (n=10, 20, 3), with significant differences between each other (P<0. 05). The HAase levels were significantly elevated in patientswith axillary lymph nodemetastasis (8. 27±3. 26mU/g,n=23) as comparedwith patientswithoutmetastasis (5. 53±2. 82 mU/g, n=10) (t=-2.305, P=0. 028).②The positive rate ofMMT-9 was significantly higher in patientswith axillary lymph nodemetastasis (78. 3%, 18/23) than patientswithoutmetastasis (30·0%, 3/10) (P=0. 016).③The expression ofHAase was significantly greater inMMT-9 positive patients (8. 57±3. 12 mU/g, n=21) than inMMT-9 negative patients (5. 47±2. 85 mU/ g,n=12) (t=2.830, P=0. 008).　Conclusions　Both HAase and MMT-9 are associated with the invasion and metastasis of human breast cancer.

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金资助项目(编号:30070292),①(中国医学科学院中国协和医科大学肿瘤医院腹外科在读博士,北京 100021 ),②通讯作者
更新日期/Last Update: 2014-02-13