[1]夏修金　 鲍卫汉　 陈东明　 洪天配①　 郑宗梅　 张玲①　 陆爱丽②　 王淑玲②.从人胚胎胰腺中分离获得巢蛋白表达阳性的细胞[J].中国微创外科杂志,2003,03(2):174-176.
　XiaXiujin,Bao Weihan,ChenDongming,et al.Nestin-positive cells isolated from human fetal pancreases[J].Chinese Journal of Minimally Invasive Surgery,2003,03(2):174-176.
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从人胚胎胰腺中分离获得巢蛋白表达阳性的细胞()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
03
期数:
2003年2期
页码:
174-176
栏目:
基础研究
出版日期:
2003-02-28

文章信息/Info

Title:
Nestin-positive cells isolated from human fetal pancreases
作者:
夏修金　 鲍卫汉　 陈东明　 洪天配①　 郑宗梅　 张玲①　 陆爱丽②　 王淑玲②　
北京大学第三医院成形外科研究中心,北京,100083
Author(s):
XiaXiujin;  Bao Weihan;  ChenDongming;  et al.
Research Center ofPlastic Surgery, Peking UniversityThirdHospital, Beijing100083, China
关键词:
胚胎 胰腺 胰腺干细胞 巢蛋白
Keywords:
Fetus　Pancreas　Pancreatic stem cell　Nestin
分类号:
R622+.9
文献标志码:
A
摘要:
目的探讨从人胚胎胰腺组织分离巢蛋白(Nestin)阳性细胞并进行体外传代培养的方法. 方法取引产的16、18、20周人胚胎胰腺组织,分离胰岛样细胞簇(Islet-like cell clusters,ICCs),进行体外培养.用免疫组织化学和逆转录-聚合酶链反应(RT-PCR)方法检测巢蛋白、胰十二指肠同源盒基因-1(Pancreatic and duodenal homeobox gene-1, PDX-1/IPF-1)以及胰岛内分泌激素胰岛素和胰升糖素(Glucagon)的表达. 结果 ICCs经体外培养可获得巢蛋白表达阳性细胞,这种细胞可进一步形成球状细胞簇,除有巢蛋白阳性细胞外,还有PDX-1、胰岛素和胰升糖素表达阳性的细胞,并且细胞可连续传代.结论从人胚胎胰腺组织中可分离获得巢蛋白表达阳性细胞,这种细胞可在体外增殖并有向胰岛内分泌细胞分化的能力.
Abstract:
Objective　To investigate whether nestin-positive cells can be isolated from human fetal pancreases and culturedin vitro for passages.　Methods　The islet-like cell clusters (ICCs) were isolated from pancreases of induced human fetuses at 16, 18, 20 gesta- tional weeks and cultured subsequently. Immunohistochemical staining and reverse transcription-polymerase chain reaction (RT-PCR) were used to detect the expression of the nestin, pancreatic and duodenal homeobox gene-1 (PDX-1/IPF-1), islet endocrine hormone insulin and glucagon.　Results　Nestin-positive cells, which may further transform into sphere cell clusters that include PDX-1, insulin and glucagon expressing cells, aswell as nestin-positive cells, were isolated fromthe ICCsinvitroand culturedwith several passages.　Conclu- sions　The nestin-positive cells can be isolated from fetal pancreases, and may have the capability to proliferate and differentiate into islet endocrine cells.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：国家自然科学基金资助项目(批准号30170443,项目类别A)①内分泌科②（北京大学干细胞研究中心,北京,100083）　
更新日期/Last Update: 2014-06-10