[1]毕洪森①　 李健宁　 薛红宇　 张培礼②.兔自体耳软骨膜复合游离移植形成工程化软骨的实验研究[J].中国微创外科杂志,2002,02(4):244-246.
　BiHongsen,Li Jianning,XueHongyu,et al.Experimental study on efficacy of perichondrium and an acellular cartilage matrix for generating cartilage[J].Chinese Journal of Minimally Invasive Surgery,2002,02(4):244-246.
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兔自体耳软骨膜复合游离移植形成工程化软骨的实验研究()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
02
期数:
2002年4期
页码:
244-246
栏目:
基础研究
出版日期:
2002-08-31

文章信息/Info

Title:
Experimental study on efficacy of perichondrium and an acellular cartilage matrix for generating cartilage
作者:
毕洪森①　 李健宁　 薛红宇　 张培礼②　
北京大学第三医院成形科,北京,100083
Author(s):
BiHongsen; Li Jianning; XueHongyu; et al.
Department ofPlastic Surgery,Peking UniversityThirdHospital,Bei- jing100083,China
关键词:
软骨膜 脱细胞基质 复合游离移植
Keywords:
Perichondrium　Acellular matrix　Free composite graft
分类号:
R-332
文献标志码:
A
摘要:
目的探求用软骨膜复合移植的方法形成自体软骨的可行性. 方法将兔自体耳软骨膜包裹异体耳软骨脱细胞基质材料复合游离移植于兔背部皮下(Ⅰ组),并在移植物附近皮下游离移植双层耳软骨膜(Ⅱa组)及单纯脱细胞的异体耳软骨基质(Ⅱb组)作为对照.在术后3周、6周分别取标本进行研究. 结果在3周、6周时,Ⅰ组与Ⅱa组成软骨细胞的增殖变化情况差异有显著性(P<0.05),Ⅰ组比Ⅱa组成软骨细胞的增殖明显旺盛,有的已经向软骨细胞转化,并有少量细胞外基质分泌,基质内软骨囊出现.Ⅱb组无成软骨细胞及软骨囊出现. 结论自体耳软骨膜与异体耳软骨脱细胞后的基质材料复合移植促进自体软骨生成.提示该基质材料有促进软骨膜细胞分化和生长的作用.
Abstract:
Objective　To study the possibility of generating cartilage with a free composite graft of perichon- drium and matrix.　Methods　A composite graft of a free perichondrium flapwrapped around acellular auricular car- tilage matrix was implanted in the back subcutaneously of young NewZealand rabbits (GroupⅠ). As controls,double folded perichondrium free flap (GroupⅡa) and acellular auricular cartilage matrix without perichondrium cover (GroupⅡb) were implanted.　Results　3 weeks and 6 weeks after implantation , obvious differences of chondro- blasts derived from the perichondrium were observed between the GroupⅠand GroupⅡa (P<0.05), and the chon- droblasts in GroupⅠwere more productive than that in GroupⅡa ,some cells have transformed into chondrocytes with secretion of matrix , even the appearance of cartilage cysts in GroupⅠ. No chondroblasts and cartilage appeared in GroupⅡb.　Conclusions　A composite graft consisting of acellular auricular cartilage matrix in an envelop of peri- chondrium is capable of heterotopic cartilage formation,and that acellular auricullar cartilage matrix serves as inductor for the perichondrium to produce chondrogenetic cells and promotes cartilage formation were proposed.

参考文献/References:

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备注/Memo

备注/Memo:
①（秦皇岛市第一医院,066000）②（包头市第七医院,014030）
更新日期/Last Update: 2014-07-31