[1]栾保华　 薛文君　 王永前　 薛峰　 吕仁荣　 张健　 初晓莲　 马晓东　 禚莹莹.两种方法保存人体透明软骨行异种移植的实验研究[J].中国微创外科杂志,2002,02(4):241-243.
　Luan Baohua,Xue Wen- jun,Wang Yongqian,et al.A study on heterotransplant between two kinds of methods in conserving hyaline cartilage[J].Chinese Journal of Minimally Invasive Surgery,2002,02(4):241-243.
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两种方法保存人体透明软骨行异种移植的实验研究()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
02
期数:
2002年4期
页码:
241-243
栏目:
基础研究
出版日期:
2002-08-31

文章信息/Info

Title:
A study on heterotransplant between two kinds of methods in conserving hyaline cartilage
作者:
栾保华　 薛文君　 王永前　 薛峰　 吕仁荣　 张健　 初晓莲　 马晓东　 禚莹莹　
山东省立医院,山东大学临床医学院美容整形外科,济南,250021
Author(s):
Luan Baohua; Xue Wen- jun; Wang Yongqian; et al.
Department ofaesthethic plastic surgery,Shandong ProvincialHospital,Jinan250021,China.
关键词:
透明软骨 异种移植
Keywords:
heterotransplant　hyaline cartilage
分类号:
R-332
文献标志码:
A
摘要:
目的探讨不同方法保存的人体透明软骨,异种移植后软骨存活的动态变化及在整形外科应用的可行性. 方法选择48只新西兰种成年白兔,随机分为两组,每组24只,分别接受-198℃液氮冷冻和75%酒精灭活法保存的人体透明软骨移植,观察一年.术后每3个月比较两组血清IgG、IgM、IgA、C3、C4、PFB(补体B因子)含量,每月随机抽取两组各两只兔子取移植软骨块观察其外形、色泽,测重量.比较光学显微镜和透射电镜下的组织学变化. 结果两实验组各时间点IgG、IgM、IgA、C3、C4、PFB含量与术前相比无显著性差异,P＞0.05.深低温保存法软骨移植后1-2个月内重量略有增加,3个月重量开始减少,至1年达最低点.光学显微镜下观察,2个月可见新生不成熟软骨细胞.3个月时软骨开始出现退行性变,至6个月时明显加重.酒精灭活移植软骨重量无明显变化,仅在6个月时开始出现轻度退行性变.透射电镜下观察的变化规律基本与光学显微镜下的观察结果相一致.结论酒精灭活法保存的人体透明软骨用于异种移植后,外型变化小,提示其临床应用可行性优于液氮冷冻保存法.
Abstract:
Objective　To compare the effects of heterotransplant between two kinds of methods in conserving hya- line cartilage.　Methods　48 adult white rabbit of New Zealand species were divided into two groups randomly.Each groups received human hyaline cartilage preserved by methods of -198℃liguid nitrogon cryopreservtion or 75% alcohol inactivation. Serum IgG,IgM,IgA, C3、C4、PFB levels were compared each 3 months with that before operation.The cont- our,color and luster,change of histology of hyaline cartilage were examined in light microscope and electron microscope and the gravitywere weight each month.　Results　IgG,IgM,IgA,C3、C4、PFB had no significant change after transplant(P> 0·05).The weights of transplanted hyaline cartilage began to increase in 1~2 months and then decreased after 3 months in methods of liguid nitrogon cryopreservation and reached theirminimum gravity at the end of one year.Neoformative immature hyaline cartilage were observed after 2 months,slight degeneration were observed after 3 months and degeneration reached their zenith in light microscope after 6 months.The weights had no change in methods of alcohol inactivation and slight de- generation began to occur after 6 months and which were similar in electron microscope.　Conclusions　Human hyaline cartilage preserved by methods of alcohol inactivation had slight change in contour and had no rejectionwhen theywere het- erotransplanted.So it suggested that their clinical application feasibility may be better than that of liguid nitrogon cryopreser- vation.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目：山东省卫生厅立项课题(编号 1999CAIDBB2)
更新日期/Last Update: 2014-07-31