[1]刘长安　 张玲　 杨光　 管增伟　 贾廷珍.低剂量辐射对脐血单个核细胞产生IL一2活性的影响[J].中国微创外科杂志,2001,01(3):162-164.
　LIUChangan,ZHANGLing,YANGGuang,et al.The effect of low dose irradiation on the expression of hter leuktn - 2 in the supernatants of cord blood mononuclear cells[J].Chinese Journal of Minimally Invasive Surgery,2001,01(3):162-164.
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低剂量辐射对脐血单个核细胞产生IL一2活性的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
01
期数:
2001年3期
页码:
162-164
栏目:
基础研究
出版日期:
2001-03-31

文章信息/Info

Title:
The effect of low dose irradiation on the expression of hter leuktn - 2 in the supernatants of cord blood mononuclear cells
作者:
刘长安　 张玲　 杨光　 管增伟　 贾廷珍
北京大学第三医院肿瘤治疗中心北京 100083
Author(s):
LIUChangan;  ZHANGLing;  YANGGuang;  et al.
CancerTreatment Center, Peking Universi- tyThirdHospital, Beijing,100083,China.
关键词:
脐血 单个核细胞 白细胞介素2 低剂量辐射
Keywords:
Cord blood　　Interleukin-2　　Mononuclear cells　　Low dose irradiation
分类号:
R73
文献标志码:
A
摘要:
目的探讨低剂量辐射对脐血单个核细胞产生的白细胞介素2(IL-2)生物学活性的影响.方法新鲜分离的脐血单个核细胞接受62mGy的γ射线照射,在照射后不同培养时间(24h,48h,72h),应用MTT(四甲基偶氮唑盐)法,以CTLL依赖株为靶细胞检测培养上清IL-2活性.结果低剂量照射后,脐血单个核细胞上清IL-2活性随培养时间推移逐渐增幅,不同时间点比较均有显著性差异(P＜0.05).在不同培养时间(24h,48h,72h),接受低剂量辐射的脐血单个核细胞培养上清IL-2活性均显著高于未照射组(P＜0.05).结论低剂量辐射可增强脐血T淋巴细胞释放的IL-2的生物学活性,从而可能在脐血造血干细胞移植时增强移植物抗白血病(GVL)效应.
Abstract:
Objective　To study on the effect of lowdose irradiation on the biological activity of interleukin- 2 (IL-2) in the supernatants of cord blood mononuclear cells.　Methods　Freshly isolated mononuclear cells from cord blood were irradiated with 62mGy gamma ray. At different culture time (24h, 48h, 72h) after irradiation, the levels of IL-2 in the supernatantswere measured by a bioassay based on CTLL cells, which served as target cells.　 Results　The biological activity of IL-2 in the supernatants of cord blood mononuclear cells enhanced increasingly after lowdose irradiation. At different culturingtime (24h, 48h, 72h),therewere significantly higher biological activ- ity of IL-2 in the supernatants of cord blood mononuclear cells irradiated with 62mGy gamma-ray as compared with those non-irradiated(AllP<0.05).Conclusions　It is suggested that enhancement of biological activity of IL-2 released fromTlymphocytes in cord blood, whichmay contribute to the strengthening of the graft-versus- leukemia (GVL) response in cord blood stem cell transplantation, can be induced by low dose irradiation.

参考文献/References:

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更新日期/Last Update: 2014-08-22