[1]刘长安　 杨光　 贾廷珍　 管增伟　 张玲.低剂量辐射对脐血T淋巴细胞膜分子表达的影响[J].中国微创外科杂志,2001,01(2):114-116.
　Liu changan,Yang guang,Jia tingzhen et,et al.Effect of low dose irradiation on expression of membrane molecules of T lymphocytes from cord blood[J].Chinese Journal of Minimally Invasive Surgery,2001,01(2):114-116.
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低剂量辐射对脐血T淋巴细胞膜分子表达的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
01
期数:
2001年2期
页码:
114-116
栏目:
基础研究
出版日期:
2001-02-28

文章信息/Info

Title:
Effect of low dose irradiation on expression of membrane molecules of T lymphocytes from cord blood
作者:
刘长安　 杨光　 贾廷珍　 管增伟　 张玲
北京大学第三临床医学院肿瘤治疗中心北京 100083
Author(s):
Liu changan; Yang guang; Jia tingzhen et; al
Peking University Third Hospital，Beijing 100083 , China
关键词:
脐血 T淋巴细胞 膜分子 低剂量辐射 脐血移植
Keywords:
Cord blood T lymphocytes Membrane molecule Low dose irradiation Cord blood transplantation
分类号:
R73
文献标志码:
A
摘要:
目的探讨低剂量辐射对人脐血T淋巴细胞膜分子表达的影响.方法62mGyγ射线照射新鲜分离的脐血淋巴细胞,照前及照后4h、12h、24h采用单克隆抗体直接免疫荧光标记流式细胞术检测TCR、CD3、CD4、CD8分子的表达.结果低剂量辐射后,脐血淋巴细胞CD3、TCR/CD3复合物、CD4、CD8分子表达显著上调,且均在4h～24h内随时间推移而逐步增幅;CD4/CD8比值无显著性变化.结论低剂量辐射可促进脐血T淋巴细胞的成熟、活化和信号的转导,从而可能在脐血移植中加速免疫功能重建,增强移植物抗白血病反应(GVL),降低肿瘤的复发率
Abstract:
Objective To study the expression of membrane molecules of T lymphocytes of cord blood after low dose irradiation(LDI).Mefhod8 Freshly isolated lymphocytes from cord blood were irradiated with 62m卿 gamma-ray . At different time ( 4h ;12h ; 24h ) a$er irradiation , the changes of TCR `，C几令，CD, "，CDg ' cells were examined场flow cytometry with direct immunofluorescence , respectively . Results The proportion of CD3 `， TCR' /CD,，;CD, ` , CD, ' cells increased significantly after LDI , the roost obvious enhancement~noted in the 24h experimental group . There~no significant changes in the ratio of CD, to CDa .Conclusions It is suggested that expedition of the maturation, activation and signal tranaduction of T lymphocytes from cord blood can be induced场ir- radiation with 62mGy gamma-ray . The reconstruction of~functions after cord blood transplantation may be ac- celerated , enhancing the graft~leukemia ( GVL) effect and preventing the tumor from relapsing .

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更新日期/Last Update: 2014-08-22