[1]朴金兰,张幼怡①,李子健**②,等.细胞外基底刚度调控宫颈癌细胞迁移的microRNA 的筛选[J].中国微创外科杂志,2017,17(07):653-661.
 Piao Jinlan*,Zhang Youyi,Li Zijian,et al.Screening of MicroRNAs Related with Cervical Cancer Cell Migration Regulated by Basement Membrane Stiffness[J].Chinese Journal of Minimally Invasive Surgery,2017,17(07):653-661.
点击复制

细胞外基底刚度调控宫颈癌细胞迁移的microRNA 的筛选()
分享到:

《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
17
期数:
2017年07期
页码:
653-661
栏目:
新技术新方法
出版日期:
2017-07-20

文章信息/Info

Title:
Screening of MicroRNAs Related with Cervical Cancer Cell Migration Regulated by Basement Membrane Stiffness
作者:
朴金兰张幼怡①李子健**②耿力**
北京大学第三医院妇产科,北京100083
Author(s):
Piao Jinlan* Zhang Youyi Li Zijian et al.
*Department of Obstetrics and Gynecology,Peking University Third Hospital, Beijing 100083, China
关键词:
宫颈癌microRNA 芯片迁移
Keywords:
Cervical cancerMicroRNA chipMigration
文献标志码:
A
摘要:
目的通过microRNA 芯片筛选不同基底刚度影响宫颈癌细胞迁移过程中的关键microRNA 的候选者。方法用丙烯酰胺及双丙烯酰胺的不同比例配置基底刚度为1、20 kPa 的人工基底膜,在其上种植宫颈癌细胞系Siha,36 h后收取细胞提取总RNA,用microRNA芯片及qRT-PCR方法筛选2种基底刚度条件下的差异表达microRNA。结果在2种不同刚度条件下生长的宫颈癌细胞系Siha 共有173 个表达差异的microRNA,其中包括70 个表达上调的microRNA和103 个表达下调的microRNA。在宫颈癌细胞系Siha、Hela、Caski 中,检测与宫颈癌细胞迁移相关的miR-21、miR-125a、miR-75b、miR-150、miR-595、miR-218、miR-200b、miR-107、miR-183 等表达与microRNA芯片结果一致。结论不同基底刚度可引起宫颈癌细胞系Siha 的microRNA 表达谱不同,microRNA 在基底刚度调控宫颈癌细胞迁移的过程中可能具有重要作用。
Abstract:
ObjectiveTo screen differently expressed microRNAs of cervical cancer cell line Siha in different basement membrane stiffness conditions.MethodsThe Siha cells were stimulated with different stiffness of basement membrane (1 kPa and 20 kPa). The total RNA was isolated after 36 h, and the microRNA chip analysis was proceeded to screen differently expressed microRNAs.ResultsWe found 173 differently expressed microRNAs, including 70 microRNAs expressed higher and 103 microRNAs expressed lower in 20 and 1kPa. We also tested some microRNAs (miR-21, miR-125a, miR-75b, miR-150, miR-595, miR-218, miR-200b, miR-107, and miR-183) closely related with migration of cervical cancer cells using real-time PCR, and the results were also in accordance with the results of microRNA chip analysis.ConclusionsCervical cancer cells have differently expressed microRNA profiles in different stiffness of basement membranes. The microRNAs may play important roles in the regulation of cervical cancer cell migration by different basement membranes.

参考文献/References:

[1]李雪,孔为民,韩超,等.首都医科大学附属北京妇产医院1992 至2011 年间宫颈癌发病趋势分析.中华妇产科学杂志,2013,6(9):310-314.
[2]李晓兰.宫颈癌发病年轻化趋势分析.中国妇幼保健,2007,22(23):3206-3207.
[3]Torre LA, Bray F, Siegel RL, et al. Global cancer statistics,2012. CA Cancer J Clin,2015,65(2):87-108.
[4]张燕茹.宫颈癌防治研究进展.中国肿瘤,2015,24(12):998-1002.
[5]王临虹,邱琇,郑睿敏,等.我国宫颈癌流行病学状况及防治策略的回顾与展望.中国妇幼卫生杂志,2010,1(3):146-149.
[6]Lee JW, Choi CH, Choi JJ, et al. Altered microRNA expression in cervical carcinomas. Clin Cancer Res,2008,14(9):2535-2542.
[7]Rao Q, Shen Q, Zhou H, et al. Aberrant microRNA expression in human cervical carcinomas. Med Oncol,2012,29(2):1242-1248.
[8]Tang J, Li Y, Wang J, et al. Molecular mechanisms of microRNAs in regulating epithelial-mesenchymal transitions in human cancers. Cancer Lett,2016,371(2):301-313.
[9]Neth P, Nazari-Jahantigh M, Schober A, et al. MicroRNAs in flow-dependent vascular remodelling. Cardiovascular Res,2013,99(2):294-303.
[10]Yehya N, Yerrapureddy A, Tobias J, et al. MicroRNA modulate alveolar epithelial response to cyclic stretch. BMC Genomics,2012,13:154.
[11]Valastyan S, Weinberg RA. Roles for microRNAs in the regulation of cell adhesion molecules. J Cell Sci,2011,124(Pt 7):999-1006.
[12]Mouw JK, Yui Y, Damiano L, et al. Tissue mechanics modulate microRNA-dependent PTEN expression to regulate malignant progression. Nat Med,2014,20(4):360-367.
[13]Nagelkerke A, Bussink J, Rowan AE, et al. The mechanical microenvironment in cancer: How physics affects tumors. Semin Cancer Biol,2015,35(12):62-70.
[14]Peralta-Zaragoza O, Deas J, Meneses-Acosta A, et al. Relevance of miR-21 in regulation of tumor suppressor gene PTEN in human cervical cancer cells. BMC Cancer, 2016,16(1):1-16.
[15]Qin X, Wan Y, Wang S, et al.MicroRNA-125a-5p modulates human cervical carcinoma proliferation and migration by targeting ABL2. Drug Des Devel Ther,2015,24(10):71-79.
[16]Yao J, Deng B, Zheng L, et al. miR-27b is upregulated in cervical carcinogenesis and promotes cell growth and invasion by regulating CDH11 and epithelial-mesenchymal transition. Oncol Rep,2016,35(3):1645-1651.
[17]Li J, Hu L, Tian Cet al. microRNA-150 promotes cervical cancer cell growth and survival by targeting FOXO4. BMC Mol Biol,2015,16(24):1-9.
[18]Zhou JY, Zheng SR, Liu J, et al. MiR-519d facilitates the progression and metastasis of cervical cancer through direct targeting Smad7. Cancer Cell Int,2016,16(21):62-70.
[19]Fornari F, Ferracin M, Trerè D, et al. Circulating microRNAs, miR-939, miR-595, miR-519d and miR-494, Identify Cirrhotic Patients with HCC. PLoS One,2015,10(10):1-15.
[20]汤贝贝, 刘水逸, 魏礼清,等.miR-218表达水平与宫颈癌侵袭转移及预后的关系.广东医学,2015,36(14):2195-2197.
[21]Cheng YX, Chen GT, Chen C. MicroRNA-200b inhibits epithelial-mesenchymal transition and migration of cervical cancer cells by directly targeting RhoE. Mol Med Rep,2016,13(4):3139-3146.
[22]张靖宜,孙燕.microRNA-506在不同肿瘤中作用的研究进展.中国肿瘤临床,2016,43(3):120-124.
[23]Che LF, Shao SF, Wang LX, et al. Downregulation of CCR5 inhibits the proliferation and invasion of cervical cancer cells and is regulated by microRNA-107. Exp Ther Med,2016,11(2):503-509.
[24]Fan D, Wang Y, Qi P, et al. MicroRNA-183 functions as the tumor suppressor via inhibiting cellular invasion and metastasis by targeting MMP-9 in cervical cancer. Gynecol Oncol,2016,141(1):166-174.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金(项目编号:81472429,81471893,81270157);973 课题计划(项目编号:2013CB933702,2014CBA02003)**通讯作者,E-mail:lzjgy1995@163.com①心内科②血管医学研究所
更新日期/Last Update: 2017-09-21