[1]刘扬,李贵斌**,王春燕,等.幼兔CO2人工气腹模型的建立[J].中国微创外科杂志,2018,18(2):148-150.
　Liu Yang,Li Guibin,Wang Chunyan,et al.Establishment of Laparoscopic Carbon Dioxide Pneumoperitoneum Model in Young Rabbits[J].Chinese Journal of Minimally Invasive Surgery,2018,18(2):148-150.
点击复制

幼兔CO2人工气腹模型的建立()
分享到：

《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
18
期数:
2018年2期
页码:
148-150
栏目:
实验研究
出版日期:
2018-03-30

文章信息/Info

Title:
Establishment of Laparoscopic Carbon Dioxide Pneumoperitoneum Model in Young Rabbits
作者:
刘扬; 李贵斌**; 王春燕; 邱云
天津市第五中心医院小儿外科，天津300450
Author(s):
Liu Yang;  Li Guibin;  Wang Chunyan;  et al.
Department of Pediatric Surgery, Fifth Central Hospital of Tianjin, Tianjin 300450, China
关键词:
腹腔镜手术; CO2气腹; 幼兔; 新生儿
Keywords:
Laparoscopic surgery; CO2 pneumoperitoneum; Young rabbit; Neonate
文献标志码:
A
摘要:
目的构建一种实用稳定的CO2人工气腹动物模型，模拟新生儿腹腔镜手术环境。方法32只健康新西兰幼兔（3周龄，体重0.8~1 kg），随机分为4组，每组8只：对照组，单纯腹腔麻醉；开腹组，麻醉后行开腹手术暴露腹腔；低压气腹组，腹腔气腹压力维持于6 mm Hg；高压气腹组：腹腔气腹压力维持于12 mm Hg。采用10%水合氯醛3 ml/kg腹腔麻醉，追加采用4%水合氯醛1 ml/kg。麻醉时间持续4 h。气腹组采用精密电子压力计控制CO2输入的方法维持目标气腹压力。结果应用精密电子压力计可以维持理想的气腹压力。各组幼兔围手术期心率、呼吸频率比较均无统计学差异（P>0.05），术后24 h内进食量恢复正常，术后7 d切口拆线，均一期愈合，未出现感染、出血及死亡。结论采用幼兔作为动物模型、精密电子压力计维持CO2气腹压力的方法，可成功建立用于研究新生儿腹腔镜手术的CO2人工气腹模型。
Abstract:
ObjectiveTo establish a practical and stable model of CO2 pneumoperitoneum and to provide experimental basis for clinical laparoscopic surgery in infants by simulating the newborn laparoscopic environment.MethodsThirtytwo healthy young rabbits were randomly divided into four groups, which were control group, open group (abdomen surgery after anesthesia), pneumoperitoneum low pressure group (abdominal pressure maintained at 6 mm Hg) and pneumoperitoneum high pressure group (abdominal pressure maintained at 12 mm Hg). The 10% chloral hydrate 3 ml/kg was injected in the abdomen for anesthesia, and 4% chloral hydrate 1 ml/mg was added to confirm the effect of anesthesia. The anesthesia time lasted for 4 hours. A sophisticated electronic pressure gauge was used to control the input of CO2 and maintain the target pneumoperitoneum pressure in the group of CO2 pneumoperitoneum model.ResultsApplication of precision electronic pressure gauge accurately maintained pneumoperitoneum pressure. In addition, there was no stastistically significant difference about the heart rate and respiratory rate amorg the 4 groups (P>0.05). The food intake returned normal in 24 hours after surgery. The incision was healed by first intention to remove the stitches 7 days after operation. There were no infection, hemorrhage or death occurred in all the 4 groups.ConclusionA model of artificial pneumoperitoneum for infant laparoscopic surgery can be successfully established by using rabbit as animal model and precision electronic manometer to maintain CO2 pneumoperitoneum pressure.

参考文献/References:

[1]赵淑萍，于海蓉，韩艳杰.腹腔镜手术人工气腹对机体的影响及护理干预.中华医院感染学杂志，2011，21（11）：2378-2380.
[2]李索林，徐伟立，韩新峰.腹腔镜技术在新生儿和小婴儿外科中的应用.中国微创外科杂志，2004，4（5）：370-372.
[3]谢涛，肖金苗.腹腔镜手术不同气腹压力对IL1β，IL6和TNFα的影响.中国微创外科杂志，2014，14（11）：1028-1032.
[4]Li LW,Zhang W,Ai YQ,et al.Influence of laparoscopic carbon dioxide pneumoperitoneum on neonate circulation and respiration.Int Med Res,2013,41(3):889-894.
[5]Kozlov Y,Novogilov V,Rasputin A,et al.Laparoscopic inguinal preperitoneal injection-novel technique for inguinal hernia repair:preliminary results of experimental study. Laparoendosc Adv Surg Tech A,2012,22:276-279.
[6]余高妍，颜崇淮，余晓刚，等.儿童慢性中度铅中毒幼兔动物模型的建立.实用儿科临床杂志，2007，22（19）：1484-1513.
[7]文建国，苏志强，王庆伟，等.幼兔逼尿肌无收缩模型的建立及影像尿动力学评估.中华小儿外科杂志，2013，34（5）：373-376.
[8]王春燕，李贵斌，刘扬，等.二氧化碳气腹对幼兔肝肾功能影响的实验研究.中华小儿外科杂志，2017，38（9）：713-717.

备注/Memo

备注/Memo:
基金项目：天津市卫计委科技基金项目（2015KZ017）；天津市滨海新区卫计委科技资金重点项目（2015BWKZ002）**通讯作者，E-mail：lgb109@163.com
更新日期/Last Update: 2018-05-10