[1]张进生﹡ 李玮 刘宁 渠渊 王立茹① 冯超 吴宗山 柴军 张前 刘春林 刘良颖.良性前列腺增生合并膀胱过度活动症患者膀胱黏膜毒蕈碱型胆碱能受体M2、M3亚型的表达及临床意义[J].中国微创外科杂志,2014,14(8):747-750.
　Zhang Jinsheng,Li Wei,Liu Ning,et al.Expression and Clinical Significance of Muscarinic Cholinergic Receptor M2 and M3 Subtypes in Patients with Benign Prostatic Hyperplasia and Overactive Bladder[J].Chinese Journal of Minimally Invasive Surgery,2014,14(8):747-750.
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良性前列腺增生合并膀胱过度活动症患者膀胱黏膜毒蕈碱型胆碱能受体M2、M3亚型的表达及临床意义()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
14
期数:
2014年8期
页码:
747-750
栏目:
基础研究
出版日期:
2014-08-20

文章信息/Info

Title:
Expression and Clinical Significance of Muscarinic Cholinergic Receptor M2 and M3 Subtypes in Patients with Benign Prostatic Hyperplasia and Overactive Bladder
作者:
张进生﹡ 李玮 刘宁 渠渊 王立茹① 冯超 吴宗山 柴军 张前 刘春林 刘良颖
首都医科大学附属复兴医院泌尿外科，北京100038
Author(s):
Zhang Jinsheng;  Li Wei;  Liu Ning;  et al.
Department of Urology, Fuxing Hospital of Capital Medical University, Beijing 100038, China
关键词:
良性前列腺增生; 膀胱过度活动症; 膀胱黏膜; 毒蕈碱型胆碱能受体
Keywords:
Benign prostatic hyperplasia; Overactive bladder; Bladder mucosa; Muscarinic cholinergic receptor
分类号:
R697+.32
文献标志码:
A
摘要:
目的探讨良性前列腺增生（benign prostatic hyperplasia，BPH）合并膀胱过度活动症（overactive bladder，OAB）患者膀胱黏膜毒蕈碱型胆碱能受体（muscarinic cholinergic receptor，M受体）M2、M3亚型表达水平的变化及临床意义。方法分析2012年5月~2013年3月20例BPH患者的国际前列腺症状评分(international prostate syndrome scoring，IPSS)、生活质量评分（quality of life，QOL）、膀胱过度活动症症状评分（OAB syndrome scoring，OABSS）和尿动力学检查。根据OABSS评分结果将其分为BPH组（9例）及BPH并OAB组（11例）。2组均在膀胱镜下钳取膀胱黏膜，经逆转录聚合酶链式反应(reverse transcriptionpolymerase chain reaction，RTPCR)检测M受体M2、M3亚型mRNA的表达水平。M2、M3受体mRNA表达水平采用相对于内参基因mRNA表达的相对值ΔCt值表示。比较2组M2、M3受体表达水平及相关性。结果M2、M3受体表达的ΔCt值BPH组明显低于BPH并OAB组（M2的ΔCt值：-0.154±0.641 vs. 0.562±0.762, t=-2.241, P=0.038；M3的ΔCt值：2.534±0.816 vs. 3.639±1.019, t=-2.630, P=0.017），说明BPH组M2、M3表达水平均高于BPH并OAB组。2组M2∶M3分别为中位数6.5206(2.35~17.33)和7.9447(2.10~23.83)（Z=-1.102，P=0.271）。同组比较，2组M2表达水平均高于M3（t=-7.776，P=0.000；t=-8.018, P=0.000）。M3表达水平与OABSS和QOL评分呈负相关（r=-0.466，P=0039；r=-0.496，P=0.026)。结论BPH合并OAB症状的患者，其膀胱黏膜M2、M3表达水平下降，且M2∶M3比例在合并OAB后有升高的趋势，在OAB发病中起一定作用。M3表达的下降，加重了OAB的程度，降低了患者的生活质量。
Abstract:
ObjectiveTo investigate the changes and significance of the expression of the M2 and M3 receptors of the urinary bladder mucosa in patients with benign prostatic hyperplasia (BPH) and overactive bladder (OAB).MethodsFrom May 2012 to February 2013, 20 cases of BPH were studied on the International Prostate Syndrome Scoring (IPSS), Quality Of Life (QOL), OAB syndrome scoring (OABSS), and urodynamics. According to OABSS results, the patients were divided into either BPH group (n=9) or BPH and OAB group (n=11). Mucosa biopsies under the cystoscopy were performed in all the cases. The expression of M2 & M3 receptors was assayed by reverse transcriptionpolymerase chain reaction (RTPCR). The mRNA expression levels of M2 & M3 receptors were indicated by the ΔCt values.ResultsThe levels of ΔCt of M2 & M3 receptors were significantly lower in the BPH group than those in the BPH+OAB group, respectively ［M2: (-0.154±0.641) vs. (0.562±0.762), t=-2.241, P=0.038; M3: (2.534±0.816) vs. (3.639±1.019), t=-2.630, P=0.017］, which implied that the expression of M2 and M3 receptors was higher in the BPH group than that in the BPH+OAB group. The median ratios of M2∶M3 in both groups were respectively 6.5206 (2.35-17.33) and 7.9447 (2.10-23.83) (Z=-1.102, P=0.271). In the same group, the expression of M2 was higher than M3 in both groups (t=-7.776, P=0.000; t=-8.018, P=0.000). The expression of M3 receptors was negatively correlated to OABSS (r=-0.466, P=0.039) and QOL (r=-0.496, P=0.026).ConclusionsThe expression of M2 and M3 receptors is decreased and the ratio of M2∶M3 is increased in patients with BPH and OAB, which may play an important role in the development of OAB. The decreasing of expression of M3 receptor exacerbates the OAB degree and reduces the quality of patients’ life.

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备注/Memo

备注/Memo:
基金项目：首都市民健康项目培育（Z131100004013013）﹡通讯作者，Email:zjs963@126.com①血液科
更新日期/Last Update: 2015-06-04