[1]于亭亭,陈霞,李昌阳,等.长期迷走神经电刺激对肥胖鼠摄食和体重的影响[J].中国微创外科杂志,2012,12(1):79-82.
　Yu Tingting,Chen Xia,Li Changyang,et al.Effect of Longterm Vagal Stimulation on Food Intake and Body Weight of Dietaryinduce Obesity in Rats[J].Chinese Journal of Minimally Invasive Surgery,2012,12(1):79-82.
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长期迷走神经电刺激对肥胖鼠摄食和体重的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
12
期数:
2012年1期
页码:
79-82
栏目:
实验研究
出版日期:
2012-01-25

文章信息/Info

Title:
Effect of Longterm Vagal Stimulation on Food Intake and Body Weight of Dietaryinduce Obesity in Rats
作者:
于亭亭; 陈霞; 李昌阳; 高兴亚; 范志宁
南京医科大学第二附属医院消化医学中心南京医科大学消化内镜研究所，南京210011
Author(s):
Yu Tingting;  Chen Xia;  Li Changyang;  et al.
Gastroenterology Center, Second Affiliated Hospital, Nanjing Medical University, Nanjing 210011, China
关键词:
肥胖; 迷走神经电刺激; 体重; 摄食; 摄食神经肽
Keywords:
Obesity; Vagal nerve stimulation; Body weight; Food intake; Ingestion neuropeptides
分类号:
R-332
文献标志码:
A
摘要:
目的观察膈下迷走神经刺激（vagal nerves stimulation，VNS）对肥胖鼠摄食、体重、摄食神经肽的影响。方法将18只雄性营养性肥胖鼠（dietary induced obesity，DIO）随机分3组：对照组、假手术组、VNS组。VNS组大鼠体内植入自主研发的闭环芯片并高脂饲养90天，芯片电刺激膈下迷走神经；假手术组体内植入不工作的芯片；对照组不做手术。术后每天测量所有动物体重、日进食量。90天后处死所有动物，分离并称重双侧附睾脂肪垫。反转录PCR法（RTPCR）半定量检测摄食神经肽：重组人刺鼠色蛋白相关蛋白（AgRP）、神经肽Y（NPY）、可卡因-苯丙胺调节转录因子编码的肽（CART）、阿片促黑色素原（POMC）的含量。结果VNS组大鼠体重增加量［(124.3±15.5)g vs. (263.1±16.8)g,P=0.013］，总摄食量［(1998.7±37.2)g vs. (3312.6±40.9)g,P=0.021］以及附睾脂肪垫重量［(15.9±2.3)g vs. (22.7±2.6)g,P=0.035］明显低于假手术组。VNS组大鼠下丘脑促摄食神经肽AgRP（P=0.027）、NPY的含量(P=0.019)低于假手术组，而抑制摄食的神经肽POMC高于假手术组（P=0.011）。结论膈下迷走神经电刺激可显著减少大鼠摄食量，减轻体重。
Abstract:
ObjectiveTo assess the effects of vagal nerve stimulation (VNS) on the food intake, body weight and level of ingestion neuropeptides in obesity rats. MethodsEighteen male dietaryinduced obesity（DIO) rats were randomly divided into control, pseudosurgery and VNS groups. In the VNS group, all the animals underwent implantation of a vagal nerve stimulator at the gastroesophogeal junction and received highfat dietary for 90 days at the mean time of the stimulation. Pseudosurgery group underwent the same procedure but without implating the stimulator. And the control received no surgery. After the surgery, the body weight and daily food intake were measured every day, and we killed the animals on day 90 to separate and weigh the bilateral epididymal fat pads. RTPCR was employed to determined the levels of ingestion neuropeptides, AgRP, NPY, CART, and POMC.ResultsVNS group showed significantly lower body weight gain ［(124.3±15.5) g vs. (263.1±16.8) g, P=0.013］, weight of epididymal fat pad ［(15.9±2.3) g vs. (22.7±2.6) g, P=0.035］, and food consumption ［(1998.7±37.2) g vs. (3312.6±40.9) g, P=0.021］;VNS group showed significantly lower level of AgRP (P=0.027) and NPY(P=0.019), but higher level of POMC (P=0.011) than the pseudosurgery group. ConclusionVNS can significantly reduce the food intake of obesity rats and thus decrease body weight.

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备注/Memo

备注/Memo:
范志宁通讯作者，Email:fanzhining@hotmail.com
更新日期/Last Update: 2013-04-11