[1]宋泉生,王晶莹,朱静琳,等.辛伐他汀对骨髓源性内皮祖细胞动员及迁移的影响[J].中国微创外科杂志,2009,09(12):1152-1155.
　Song Quansheng,Wang Jingying,Zhu Jinglin,et al.Effect of Simvastatin on Mobilization and Migration of Endothelial Progenitor Cells[J].Chinese Journal of Minimally Invasive Surgery,2009,09(12):1152-1155.
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辛伐他汀对骨髓源性内皮祖细胞动员及迁移的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
09
期数:
2009年12期
页码:
1152-1155
栏目:
实验研究
出版日期:
2009-12-31

文章信息/Info

Title:
Effect of Simvastatin on Mobilization and Migration of Endothelial Progenitor Cells
作者:
宋泉生; 王晶莹; 朱静琳; 韩晓光; 李旭; 杨燕琳①; 孙艳①; 宋纯理**
北京大学第三医院骨科，北京100191
Author(s):
Song Quansheng;  Wang Jingying;  Zhu Jinglin;  et al.
Department of Orthopaedics, Peking University Third Hospital, Beijing 100191, China
关键词:
辛伐他汀; 内皮祖细胞; 动员; 迁移
Keywords:
Simvastatin; Endothelial progenitor cells; Mobilization; Migration
分类号:
R-332
文献标志码:
A
摘要:
目的研究辛伐他汀对兔内皮祖细胞（endothelial progenitor cells，EPCs）迁移、动员的影响。方法将2只兔从髂骨抽取骨髓，采用贴壁法，在条件培养基M199中培养EPCs，并用免疫荧光法鉴定CD34、CD133、VEGFR2。经鉴定的EPCs，在不同浓度辛伐他汀（分别为0.01，0.1，1.0 μmol/L）作用下，Transwell实验观察辛伐他汀对EPCs迁移的影响。6只兔随机分为实验组和对照组，每组3只。6只兔颅骨造极限骨缺损模型，实验组和对照组在颅骨缺损处分别植入辛伐他汀复合聚乳酸薄饼或聚乳酸薄饼，术后10天，取外周血，流式细胞仪检测CD34/CD133双阳性细胞表达率，观察辛伐他汀对EPCs动员的效果。 结果Transwell实验，辛伐他汀作用于EPCs 16小时，0.1 μmol/L和1.0 μmol/L辛伐他汀组细胞迁移数量OD值（0.097±0.011，0.099±0.019）较0.01 μmol/L辛伐他汀组（0.075±0.013）与对照组（0.077±0.014）多，差异有显著性（P<0.05)。造模术后10天，辛伐他汀组3只兔外周血中CD34+/CD133+细胞表达率为0.28%，0.84%，028%，对照组为026%，0.11%，0.09%。结论辛伐他汀可动员EPCs到外周血，可提高骨髓源性EPCs的迁移能力。
Abstract:
ObjectiveTo investigate the effect of simvastatin on the mobilization and migration of endothelial progenitor cells (EPCs). MethodsEPCs were harvested from the bone marrows of two rabbits, cultured with M199, and identified by immunohistochemistry. The identified EPCs were then treated with simvastatin with different concentrations (0, 0.01, 0.1, 1.0 μmol/L),and their migration induced by simvastatin was determined with Transwell chamber assay. Six rabbits models of cranial bone defect were established and divided into control and experiment groups (3 in each). In order to elicit the effects of simvastatin on mobilization of EPCs, simvastatin was embedded in polylactic acid compound, and implanted into the cranial bone defect area in the experiment group. Meanwhile, polylactic acid was implanted in the control animals. After 10 days, the expression rate of CD34+/CD133+ EPCs in the rabbit peripheral blood was counted by flow cytometry to determine the motivating effect of simvastatin.ResultsIn Transwell experiment, 16 hours after adding simvastatin (0, 0.01, 0.1 or 1.0 μmol/L), the cell migration ability was obviously increased showing a dosedependent trend (OD value: 0.077±0.014 in control group and 0.075±0.013 in 0.01 μmol/L group vs 0.097±0011 in 0.1 μmol/L group and 0.099±0.019 in 1.0 μmol/L group, P<0.05). In animal experiment, 10 days after the implantation of simvastatin, the expression rate of CD34+/CD133+ EPCs were 0.28%, 0.84%, and 0.28% respectively in the three rabbits, while in the control groups, the rates were 0.26%, 0.11% and 0.09%.ConclusionSimvastatin could mobilize EPCs into peripheral blood, and improve migration capability of EPCs.

参考文献/References:

[1]Song CL,Guo ZQ,Ma QJ,et al.Simvastatin induces osteoblastic differentiation and inhibits adipocytic differentiation in mouse bone marrow stromal cells.Biochem Biophys Res commun,2003,308:458-462.
[2]Song CL,Wang JY,Song QS,et al.Simvastatin induces estrogen receptoralpha (ERα) in murine bone marrow stromal cells.J Bone Miner Metab,2008,26:213-217.
[3]Asahara T,Murasawa S.Endothelial progenitor cells for vasculogenesis.Physiology,2005,20:36-42.
[4]Kawamoto A,Tkebuchava T,Yamaguchi J,et al.Intramyocardial transplantation of autologous endothelial progenitor cells for therapeutic neovascularization of myocardial ischemia.Circulation,2003,107:461-468.
[5]侯锐，毛天球，杨耀武，等.构建组织工程骨修复兔颅骨极限缺损的实验研究.中国修复重建外科杂志,2005,19:818-820.
[6]Daniel JC,Geoffrey CG.Homing to hypoxia:HIF1 as a mediator of progenitor cell recruitment to injured tissue.Trends Cardiovasc Med,2005,15:57-63.
[7]Whittaker A,Moore JS,VasaNicotera M,et al.Evidence for genetic regulation of endothelial progenitor cells and their role as biologicalmarkers of atherosclerotic susceptibility.Eur Heart J,2008,29(3):332-338.
[8]Kim SY,Park SY,Yang J H,et al.Differentiation of endothelial cells from human umbilical cord blood AC133+CD14+ cells.Ann Hematol,2005,84(7):417-422.
[9]Iwaguro H,Asahara T.Endothelial progenitor cell culture and gene transfer.Met hods Mol Med,2005,112:239-247.
[10]Bjorn S,Per A.Locally applied Simvastatin improves fracture healing in mice.BMC Musculoskelet Disord,2007,8:98-103.

备注/Memo

备注/Memo:
基金项目：国家自然科学资金资助项目（30300352,30772200），**通讯作者，①中心实验室
更新日期/Last Update: 2014-01-08