[1]李枫①陈东明※,乔晋琳②,孙东杰,等.小针刀对移植于裸鼠的人皮肤增生性瘢痕组织中Ⅰ、Ⅲ型胶原蛋白的影响[J].中国微创外科杂志,2009,09(11):1038-1041.
　Li Feng,Chen Dongming*,Qiao Jinlin,et al.Effect of Small NeedleKnife on Collagens Ⅰ and Ⅲ in Transplanted Human Skin Hypertrophic Scar Tissues in Nude Mice[J].Chinese Journal of Minimally Invasive Surgery,2009,09(11):1038-1041.
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小针刀对移植于裸鼠的人皮肤增生性瘢痕组织中Ⅰ、Ⅲ型胶原蛋白的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
09
期数:
2009年11期
页码:
1038-1041
栏目:
基础研究
出版日期:
2009-11-30

文章信息/Info

Title:
Effect of Small NeedleKnife on Collagens Ⅰ and Ⅲ in Transplanted Human Skin Hypertrophic Scar Tissues in Nude Mice
作者:
李枫①陈东明※; 乔晋琳②; 孙东杰; 唐岩①; 唐军民①; 赵霞; 毕振伍①; 梁艳清③
北京大学第三医院成形科，北京100191
Author(s):
Li Feng;  Chen Dongming*;  Qiao Jinlin;  et al.
*Department of Plastic Surgery, Peking University Third Hospital, Beijing 100191, China
关键词:
小针刀; 裸鼠; 增生性瘢痕; 胶原纤维
Keywords:
Small Needleknife; Nude Mice; Hypertrophic Scars; Collagen Fibre
分类号:
R-332
文献标志码:
A
摘要:
目的探讨小针刀对移植于裸鼠皮下人增生性瘢痕真皮组织Ⅰ、Ⅲ型胶原蛋白的作用。方法将6例人无表皮的增生性瘢痕组织分别移植于24只裸鼠背部皮下，建立增生性瘢痕裸鼠动物模型。术后10天，分为对照组、0.1 mg/ml曲安奈德组、0.2 mg/ml曲安奈德组和小针刀组，每组6只，14天取材，利用免疫组织化学染色检测Ⅰ、Ⅲ型胶原蛋白的变化。结果Ⅰ、Ⅲ型胶原分布于各组成纤维细胞的胞浆及组织中。图像分析结果，0.1 mg/ml曲安奈德组和0.2 mg/ml曲安奈德组Ⅰ、Ⅲ型胶原含量（0.09±0.03，0.11±0.05；0.12±0.02，0.11±0.01）低于对照组（0.17±0.04，0.19±0.03）(P<0.05)；小针刀组Ⅰ、Ⅲ型胶原含量（0.12±0.02，0.12±0.02）与曲安奈德组差异无显著性（P>0.05）。结论小针刀可降低移植于裸鼠皮下增生性瘢痕真皮组织中Ⅰ、Ⅲ型胶原蛋白的含量。
Abstract:
ObjectiveTo discuss the effect of small needle knife therapy on collagensⅠ and Ⅲ in the hypertrophic scar tissues that was subcutaneously transplanted into nude mice. MethodsSix samples of human hypertrophic scar tissues without cuticle were subcutaneously transplanted into the back of 24 nude mice to create the animal models of hypertrophic scar. Ten days after of the operation, the mice were divided into control, 0.1 mg/ml triamcinolone, 0.2 mg/ml triamcinolone, and small needleknife groups with 6 mice in each. Specimens of the scar tissues were collected in 14 days for immunohistochemistry to detect the collagen Ⅰ and Ⅲ. ResultsIn all the groups, collagens Ⅰand Ⅲ distributed extensively in the cytoplasm of fibroblast and tissues. Image analysis showed that the concentration of collagens Ⅰand Ⅲ in 0.1 mg/ml and 0.2 mg/ml triamcinolone groups were significantly lower than those in the control (0.09±0.03， 0.11±0.05 and 0.12±0.02， 0.11±0.01 vs. 0.17±0.04， 0.19±0.03, P<005), but similar to that in the small needleknife group (0.12±0.02， 0.12±0.02, P>005). ConclusionsSmall needleknife therapy can reduce the concentration of collagens Ⅰand Ⅲ in transplanted hypertrophic scar tissues in nude mice.

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备注/Memo

备注/Memo:
※通讯作者 ①（北京大学医学部组织胚胎学教研室，北京100191）②（海军总医院康复理疗科，北京100037）③（广东医学院组织胚胎教研室，广州524023）
更新日期/Last Update: 2014-01-08