[1]张成俊,夏亚一,王常德,等.流体切应力下SIVA1凋亡诱导因子促成骨细胞增殖分化的双向调节作用[J].中国微创外科杂志,2009,09(8):741-746.
　Zhang Chengjun,Xia Yayi,Wang Changde,et al.Twoway Regulation of Apoptosisinducing Factor SIVA1 in the Proliferation and Differentiation of Osteoblasts under Fluid Shear Stress[J].Chinese Journal of Minimally Invasive Surgery,2009,09(8):741-746.
点击复制

流体切应力下SIVA1凋亡诱导因子促成骨细胞增殖分化的双向调节作用()
分享到：

《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
09
期数:
2009年8期
页码:
741-746
栏目:
基础研究
出版日期:
2009-08-31

文章信息/Info

Title:
Twoway Regulation of Apoptosisinducing Factor SIVA1 in the Proliferation and Differentiation of Osteoblasts under Fluid Shear Stress
作者:
张成俊; 夏亚一; 王常德; 汪静; 韵向东; 汉华
兰州大学第二医院骨科研究所，兰州730030
Author(s):
Zhang Chengjun;  Xia Yayi;  Wang Changde;  et al.
Institute of Orthopedics, Second Hospital Affiliated to Lanzhou University, Lanzhou 730030, China
关键词:
流体切应力; 成骨细胞; 增殖分化; SIVA1凋亡诱导因子
Keywords:
Fluid shear stress; Osteoblast; Proliferation and differentiation; Apoptosisinducing factor SIVA1
分类号:
R-332
文献标志码:
A
摘要:
目的探讨流体切应力（fluid shear stress，FSS）对KM小鼠成骨细胞增殖分化及相关细胞凋亡诱导因子（SIVA1）的调节作用。方法将传至3代成骨细胞分为应力刺激组（试验组）和非应力刺激组（对照组）。5个试验组分别给予12 Pa FSS作用0.25，0.5，1，2，4 h；对照组为0 h。MTT法测定细胞增殖，检测碱性磷酸酶（ALP）活性，半定量RTPCR测定凋亡诱导因子SIVA1的表达水平。结果FSS促增殖作用在短期（0.25，0.5 h）明显，且细胞生长曲线前移；但在1，2，4 h却有明显抑制增殖作用。FSS同样增加了ALP活性，尤其在应力作用0.5 h时显著（ALP含量为2.4320±0.205金氏单位/100 ml，相对对照达158%）(P＜0.05)；而应力作用1，2，4 h后减低了ALP的表达。应力初期SIVA1 mRNA表达量明显下降，尤其在FSS作用0.5 h后SIVA1/GAPDH相对表达量为0.099±0.002，相对对照明显下调了71.3%(P＜0.01)，此效应在作用1 h后减退，SIVA1 mRNA表达开始升高，4 h后升高明显。结论1.2 Pa FSS在短时间刺激下可刺激成骨细胞增殖分化，尤其在0.5 h后效应明显。早期促细胞增殖和ALP水平升高主要与SIVA1 mRNA表达下调有关，而后期抑制作用可能与SIVA1 mRNA表达升高有关。
Abstract:
ObjectiveTo investigate the regulatory effect of fluid shear stress (FSS) on the proliferation and differentiation of osteoblasts, as well as the expression of apoptosisinducing factor, SIVA1. MethodsThe thirdpassage osteoblasts were divided into five experiment groups and one control group. In the experiment groups, 1.2 Pa FSS were given to the osteoblasts for 0.25, 0.5, 1, 2, and 4 hours respectively, while the control group received no FSS. Afterwards, the cells were harvested to measure MTT value and ALP activity; mRNA level of SIVA1 were determined by RTPCR.ResultsMTT revealed that the cells proliferation markedly increased in the 0.25 h and 0.5 h experiment groups with advanced cell growth curve; whereas significantly inhibited in 1, 2, and 4 h groups. The FSS also increased the ALP activity at 0.25 and 0.5 hour, especially in the 0.5 h group (2.4320±0.205 S unit/100ml, 158% of the control; P＜0.05), while at the 1, 2 and 4 h groups, the expression of ALP deceased significantly. The SIVA1 mRNA expression decreased as a result of shorttime FSS, the relative expression of SIVA1/GAPDH is 0.099±0.002 at 0.5 hour (decreased by 71.3% compared with the control, P＜0.01). The expression began to increase at 1 hour, and then increased significantly at 4 hours.Conclusions1.2 Pa FSS can enhance the proliferation and differentiation of osteoblasts at the early period; the efficacy is especially significant at 0.5 hour. The enhanced proliferation and increased ALP activity at early stage is related to the downregulation of SIVA1 expression, and the inhibitory effect in the late state may be associated with the increased expression of SIVA1.

参考文献/References:

[1]Nauman EA,Satcher RL,Keaveny TM,et al.Osteoblasts respond to pulsatile fluid flow with shortterm increases in PGE2 but no change in mineralization.J Appl Physiol,2001,90:1849-1854.
[2]陈伟辉，乔鞠，罗颂椒，等.不同流动剪切力调节大鼠成骨样细胞增殖的体外研究.华西医科大学学报，2001，32(2):232-234.
[3]钱煦.应力方向对内皮细胞力学传递和功能的影响.北京大学学报(自然科学版),2007,43(4):435-440.
[4]Yamamoto K,Takahashi T,Asahara T,et al.Proliferation, differentiation, and tube formation by endothelial progenitor cells in response to shear stress.J Appl Physiol,2003，95(5):2081-2088.
[5]Datta N,Pham QP,Sharma U,et al.In vitro generated extracellular matrix and fluid shear stress synergistically enhance 3D osteoblastic differentiation.Proc Natl Acad Sci U S A,2006,103:2488-2493.
[6]Tatsumi S,Ishii K,Amizuka N,et al.Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction.Cell Metab,2007,5(6):464-475.
[7]Lin FT,Lai YJ,Makarova N,et al.The lysophosphatidic acid 2 receptor mediates downregulation of Siva1 to promote cell survival.J Biol Chem,2007,282(52):37759-37769.
[8]夏亚一,张成俊,王常德.骨细胞力学信号转导及基因表达.国际骨科学杂志,2008,29(3):182-187.
[9]Migliaccio S,Brama M,Fornari R,et al.Glucocorticoidinduced osteoporosis:an osteoblastic disease.Aging Clin Exp Res,2007,19(3 Suppl):5-10.

备注/Memo

备注/Memo:
夏亚一通讯作者
更新日期/Last Update: 2013-09-17