[1]张小青,尹刚,徐智※,等.管腔外局部应用紫杉醇对豚鼠胆管对端吻合术后吻合口愈合的作用[J].中国微创外科杂志,2008,08(8):748-751.
　Zhang Xiaoqing,Yin Gang,Xu Zhi,et al.Effect of Local Delivery of Paclitaxel on Anastomotic Wound Healing of the Common Bile Duct in Guinea Pig[J].Chinese Journal of Minimally Invasive Surgery,2008,08(8):748-751.
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管腔外局部应用紫杉醇对豚鼠胆管对端吻合术后吻合口愈合的作用()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
08
期数:
2008年8期
页码:
748-751
栏目:
出版日期:
2008-11-02

文章信息/Info

Title:
Effect of Local Delivery of Paclitaxel on Anastomotic Wound Healing of the Common Bile Duct in Guinea Pig
作者:
张小青; 尹刚; 徐智※; 凌晓锋; 王立新; 侯纯升
北京大学第三医院普通外科，北京100191
Author(s):
Zhang Xiaoqing;  Yin Gang;  Xu Zhi;  et al.
Department of General Surgery, Peking University Third Hospital, Beijing 100191, China
关键词:
胆管对端吻合; 紫杉醇; 透射电镜; 胆管狭窄; 豚鼠
Keywords:
Endtoend anastomosis; Paclitaxel; Transmission Electron Microscope; Biliary stricture; Guinea pig
分类号:
R-332
文献标志码:
A
摘要:
目的探讨局部应用紫杉醇对豚鼠动物模型胆管对端吻合术后瘢痕愈合、狭窄发生抑制作用的可能性。方法制作豚鼠胆管对端吻合动物模型。将42只豚鼠按术后时间（3 d、2周、1个月）随机分为3组，每组按紫杉醇组与对照组各分为2组，每组7只。紫杉醇组于显微吻合操作完成后吻合口管壁外局部涂抹紫杉醇溶液（1000 μmol/L，0.05 ml），作用10 min左右。术后分别于3 d、2周、1个月时取材，与对照组比较，进行组织学、管壁厚度检测。应用透射电镜观察紫杉醇诱导细胞凋亡的超微结构。结果术后胆管壁呈炎症反应，内膜增生显著。术后3天和1个月时，紫杉醇组管壁厚度均小于对照组［3天：(574.41±24.53) μm vs (802.98±24.42) μm，t=-6.604,P=0.000；1个月：(1383.36±36.64) μm vs (1518.56±34.89) μm, t=-2.672,P=0020)］。透射电镜可见紫杉醇诱导细胞凋亡及细胞器损害超微结构表现。结论管腔外局部单次使用紫杉醇1个月内可起到延缓豚鼠动物模型胆管管壁增厚的作用。
Abstract:
ObjectiveTo investigate the effect of local delivery of paclitaxel on bile duct scar formation and biliary stricture in guinea pig.MethodsModels of endtoend anastomosis of the common bile duct were established in 42 guinea pigs. The animals were randomly divided into paclitaxel and control groups. In the paclitaxel group, the drug solution (1000 μmol/L，0.05 ml) was smeared onto the anastomotic wound before closing the abdomen. The animals were killed in 3 days, 2 weeks, and 1 month after the operation (7 in each group at each time point). The sepcimens of the common bile duct were obtained for histological examination. Transmission electron microscopy was employed to observe the ultrastructure of paclitaxelinduced apoptotic cells. ResultsHistological examination showed inflammatory reaction and hyperplasia of the bile duct wall. The bile duct wall of the paclitaxel group was significantly thiner than that of the control group in 3 days and 1 month after the operation ［3 days: (574.41±24.53)μm vs (802.98±24.42) μm, t=-6.604, P=0.000; 1 month: (1383.36±36.64) μm vs (1518.56±34.89)μm, t=-2.672, P=0.020］. Paclitaxelinduced cell apoptosis and injured cell organs were found by transmission electron microscopy.ConclusionSingle local delivery of paclitaxel can inhibit biliary stricture in one month.

参考文献/References:

[1]Metzger P,Gamal EM.Bile duct injuries in the era of laparoscopic cholecystectomy.Int Surg，1995，80(4):328-331.
[2]徐智.梗阻性黄疸对器官系统功能的损害及对策.中国实用外科杂志，2007，27(10)：788-791.
[3]徐军,耿智敏,刘青光,等.碱性成纤维细胞生长因子预防胆肠吻合口狭窄的实验研究.西安医科大学学报，2002，23(2):262-264.
[4]Tez M,Keskek M,Ozkan O,et al.External metallic circle in microsurgical anastomosis of common bile duct.Am J Surg，2001，182(2):130-133.
[5]Park SM,Choi JW,Kim ST,et al.Suppression of proliferative cholangitis in a rat model by local delivery of paclitaxel.J Hepatobiliary Pancreat Surg，2003，10(2):176-182.
[6]Jordan MA,Wilson L.Microtubules and actin filaments: dynamic targets for cancer chemotherapy.Curr Opin Cell Biol，1998，10(1):123-130.
[7]SalahEldin AE,Inoue S,Tsukamoto S,et al.An association of Bcl2 phosphorylation and Bax localization with their functions after hyperthermia and paclitaxel treatment.Int J Cancer，2003，103(1):53-60.
[8]Longuet M,Serduc R,Riva C.Implication of bax in apoptosis depends on microtubule network mobility.Int J Oncol，2004，25(2):309-317.
[9]Stumm S,Meyer A,Lindner M,et al.Paclitaxel treatment of breast cancer cell lines modulates Fas/Fas ligand expression and induces apoptosis which can be inhibited through the CD40 receptor.Oncology，2004，66(2):101-111.
[10]Robinson G,Gray T.Electron microscopytissue preparation,sectioning and staining.In:Bancroft JD,Stevens A,eds.Theory and practice of histological techniques.3rd ed.London:Churchill Livingstone,1990.525-562.
[11]Bun SS,Giacometti S,Fanciullino R,et al.Effect of several compounds on biliary excretion of paclitaxel and its metabolites in guineapigs.Anticancer Drugs,2005,16(6):675-682.
[12]Cresteil T,Monsarrat B,Alvinerie P,et al.Taxol metabolism by human liver microsomes:identification of cytochrome P450 isozymes involved in its biotransformation.Cancer Res,1994,54(2):386-392.
[13]Cresteil T,Monsarrat B,Dubois J,et al.Regioselective metabolism of taxoids by human CYP3A4 and 2C8:structureactivity relationship.Drug Metab Dispos,2002,30(4):438-445.
[14]Bun SS,Ciccolini J,Bun H,et al.Drug interactions of paclitaxel metabolism in human liver microsomes.J Chemother,2003,15(3):266-274.
[15]Groschel B,Bushman F.Cell cycle arrest in G2/M promotes early steps of infection by human immunodeficiency virus.J Virol,2005,79(9):5695-5704.
[16]Heldman AW,Cheng L,Jenkins GM,et al.Paclitaxel stent coating inhibits neointimal hyperplasia at 4 weeks in a porcine model of coronary restenosis.Circulation,2001,103(18):2289-2295.
[17]Dawkins KD,Grube E,Guagliumi G,et al.TAXUS VI investigators clinical efficacy of polymerbased paclitaxeleluting stents in the treatment of complex, long coronary artery lesions from a multicenter,randomized trial:support for the use of drugeluting stents in contemporary clinical practice.Circulation,2005,112(21):3306-3313.
[1８]Lee DK,Kim HS,Kim KS,et al.The effect on porcine bile duct of a metallic stent covered with a paclitaxelincorporated membrane.Gastrointest Endosc,2005,61(2):296-301.
[19]李永林.瘢痕挛缩机理的研究和生物学治疗进展.国外医学·创伤与外科基本问题分册,1989,10(5):7-9.
[20]Desmoulière A,Redard M,Darby I,et al.Apoptosis mediates the decrease in cellularity during the transition between granulation tissue and scar.Am J Pathol,1995,146(1):56-66.
[21]Rowinsky EK,Donehower RC.Paclitaxel (taxol).N Engl J Med,1995,332(15):1004-1014.

备注/Memo

备注/Memo:
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更新日期/Last Update: 2013-10-23