[1]崔赓,李洁①,雷伟*,等.一种新型注射型骨修复材料对犬桡骨骨缺损修复的实验研究[J].中国微创外科杂志,2008,08(8):735-738.
　Cui Geng*,Li Jie,Lei Wei*,et al.Injectable Osteoinductive Material with Fibrin Sealant as a Carrier for Repairing Radial Defect in Dogs[J].Chinese Journal of Minimally Invasive Surgery,2008,08(8):735-738.
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一种新型注射型骨修复材料对犬桡骨骨缺损修复的实验研究()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
08
期数:
2008年8期
页码:
735-738
栏目:
实验研究
出版日期:
2008-11-02

文章信息/Info

Title:
Injectable Osteoinductive Material with Fibrin Sealant as a Carrier for Repairing Radial Defect in Dogs
作者:
崔赓; 李洁① ; 雷伟* ; 胡蕴玉; 吕荣; 唐佩福②;  梁雨田②; 陶笙②; 张群②; 郭义柱②; 梁向党②; 王岩②
第四军医大学西京医院骨科医院，西安710032
Author(s):
Cui Geng*;  Li Jie;  Lei Wei*;  et al.
*Institute of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
关键词:
纤维蛋白胶; 骨形态发生蛋白; 碱性成纤维细胞生长因子; 骨缺损; 修复
Keywords:
Fibrin sealant; Bone morphogenetic protein; Basic fibroblast growth factor; Bone defect; Repair
分类号:
R-332
文献标志码:
A
摘要:
目的探讨以纤维蛋白胶（fibrin sealant，FS）为载体复合牛骨形态发生蛋白（bBMP）和碱性成纤维细胞生长因子（bFGF）的注射型骨修复材料修复犬桡骨节段性缺损的效果。方法12只家犬随机分为实验组和对照组，每组6只。12只成年健康家犬右侧桡骨中上段造成2.0 cm缺损，制成犬桡骨2.0 cm骨缺损实验模型，然后严密缝合皮下组织及皮肤。对照组和实验组分别经切口周围正常皮肤注射FS和FS＋bFGF＋bBMP到骨缺损处，术后4、8、16、24周进行放射学检查，术后24周取标本进行组织学和骨密度检查，研究其成骨效应。结果①影像学：实验组术后4周缺损区有密度较低的骨痂形成；术后24周，皮质完全连接，髓腔再通。对照组术后24周内缺损区均无骨痂生长，骨缺损未得到修复。②骨密度测定：术后24周时实验组的骨密度（456.33±13.74）mg/cm2显著高于健侧（433.33±6.77）mg/cm2（t＝2.57，P＝0.00）及对照组(0 mg/cm2)的骨密度。③组织形态学：术后24周实验组新生皮质骨十分致密，骨皮质连接完整，髓腔完全再通；对照组无新生骨形成，缺损处完全为结缔组织修复。结论以FS为载体复合bBMP和bFGF的注射型骨修复材料具有高效的骨修复能力，对家犬的骨缺损有良好的修复效果。
Abstract:
ObjectiveTo investigate the efficacy of injectable osteoinductive material with fibrin sealant (FS) as a carrier compounded with bovine bone morphogenetic protein (bBMP) and bovine fibroblast growth factor (bFGF) for radial defect in dogs.MethodsA total of 12 dogs were used in this study. The animals were randomly divided into treatment and control groups with 6 in each. We created a 20mm bone defect at the upper radius of each dog, and then sutured the subcutaneous tissues and skin around the lesion. After the operation, FS (control) and FS+bFGF+bBMP were given to the two groups respectively by percutaneous injection. To compare the efficacy of the injections, we examined the animals by radiography in 4, 8, 16, and 24 weeks. The dogs were sacrificed in 24 weeks to obtain the specimens of the bone defect for histological examination and bone mineral density (BMD) determination.ResultsRadiography showed callus formation in 4 weeks and then osteoneogenesis in 24 weeks in the treatment group; whereas, in the control group, no callus was found around the defect in 24 weeks. In the treatment group, the mean BMD of the diseased radius was significantly higher than that of the healthy leg and that in the control group ［(456.33±13.74) mg/cm2 vs (43333±677) mg/cm2 (t＝2.57, P＝0.00) and 0 mg/cm2］. By histological examination, the newformed bone in the treatment group was confirmed integral and dense with intact cortex and continuous marrow cavities in 24 weeks, while the bone defect of the controls were repaired with connective tissues without remodeling of the bone. ConclusionIt is effective to repair bone defect in dogs by using injectable osteoinductive material with FS as a carrier compounded with bBMP and bFGF.

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备注/Memo

备注/Memo:
①(解放军总医院妇产科，北京100853)②(解放军总医院骨科，北京100853)*通讯作者
更新日期/Last Update: 2013-10-23