[1]郝迎学,钟华①,张超,等.体外模拟气腹环境对胃癌细胞增殖凋亡的影响[J].中国微创外科杂志,2008,08(6):552-555.
　Hao Yingxue*,Zhong Hua,Zhang Chao*,et al.Effects of Simulated Pneumoperitoneum on Proliferation and Apoptosis of Gastric Cancer Cells[J].Chinese Journal of Minimally Invasive Surgery,2008,08(6):552-555.
点击复制

体外模拟气腹环境对胃癌细胞增殖凋亡的影响()
分享到：

《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
08
期数:
2008年6期
页码:
552-555
栏目:
基础研究
出版日期:
2008-10-31

文章信息/Info

Title:
Effects of Simulated Pneumoperitoneum on Proliferation and Apoptosis of Gastric Cancer Cells
作者:
郝迎学; 钟华①; 张超; 曾冬竹; 石彦; 饶芸; 余佩武**
第三军医大学西南医院普通外科、微创胃肠外科中心，重庆400038
Author(s):
Hao Yingxue*;  Zhong Hua;  Zhang Chao*;  et al.
*Department of General Surgery and Center of Microinvasive Gastrointestinal Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
关键词:
气腹; 胃癌细胞; 增殖; 凋亡
Keywords:
Pneumoperitoneum;  Gastric cancer cells;  Proliferation;  Apoptosis
分类号:
R735.2
文献标志码:
A
摘要:
目的通过体外模拟气腹环境，观察二氧化碳（CO2）和氦气（He）在不同压力下对胃癌细胞MKN45增殖、凋亡的影响。方法实验分为对照组、CO2组和He组，其中CO2组和He组又按照压力不同分为0、5、10、15 mm Hg组（作用时间均为4 h）。细胞在密闭培养箱内经不同压力CO2或He作用4 h后用血气分析仪检测培养液pH值；MTT法检测细胞增殖情况；流式细胞仪观察细胞凋亡指数变化；Annexin VFITC/PI双标染色、流式细胞仪测定凋亡细胞比例。结果处理结束后即刻检测，CO2组细胞培养液呈弱酸性，He组细胞培养液呈弱碱性。MTT法检测细胞增殖变化，CO2组0、5、10 mm Hg组OD值与对照组比较无明显差异（P＞0.05）,15 mm Hg组OD值与对照组比较在前3天无明显差异（P＞0.05），在4~7天OD值明显低于对照组（P＜0.01）。而在He组，不同压力组细胞增殖活性与对照组比较无明显差异（P＞0.05）。在CO2环境下，0、5 mm Hg组细胞凋亡指数与对照组比较无明显差异（P＞0.05），而10、15 mm Hg组明显大于正常对照组（P＜0.01）。0、5 mm Hg组细胞凋亡比例与对照组比较无显著差异（P＞0.05），而10、15 mm Hg组细胞凋亡比例明显大于对照组（P＜0.01）。在He环境下0、5、10、15 mm Hg组细胞凋亡指数和凋亡比例较对照组均无明显差异（P＞0.05）。结论在临床常用压力范围内（≤10 mm Hg），CO2对胃癌细胞增殖、凋亡无明显影响，而在15 mm Hg压力下，CO2抑制细胞的增殖。
Abstract:
ObjectiveTo investigate the effects of CO2 and helium insufflation administered at different pressures on the proliferation and apoptosis of cultured human gastric cancer cells, MKN45.MethodsThe gastric cancer cells MKN45 were divided into control, CO2, and helium groups. In CO2 and helium groups, the cells were exposed to CO2 or helium at different pressures (0, 5, 10, and 15 mm Hg) for 4 hours. And then pH of each culture media in the three groups was analyzed, the proliferation of the cells was detected by MTT assay, and the apoptotic index （AI） of each group was determined by flow cytometry. The cells were double labeled by AnnexinⅤFITC/PI staining, and the proportion of apoptotic cells in each group was tested by flow cytometry.ResultsThe cell media was weak acid in the CO2 group, and was weak basic in the helium group. MTT assay showed that the OD value of the 0, 5, and 10 mm Hg subgroups in the CO2 group was not significantly different from that in the control (P＞0.05). Whereas, in the 15 mm Hg subgroup of the CO2 group, the OD value was similar to that of the control during the first 3 days (P＞0.05), but decreased significantly from 4 to 7 days (P＜0.01). The OD value of each subgroup of the helium group was similar to that of the control (P＞0.05). In the CO2 group, the AI and proportion of apoptotic cells of the 0 and 5 mm Hg subgroups were not significantly different from those of the control (both P＞0.05), while those of the 10 and 15 mm Hg subgroups were significantly higher than that of the control (both P＜0.01). The AI and proportion of apoptotic cells in the helium group were similar to those of the control (both P＞0.05). ConclusionsA pressure of CO2 ≤10 mm Hg do not influence the proliferation and apoptosis of gastric cancer MKN45 cells. When the pressure reach 15 mm Hg, the proliferation of the cells is inhibited.

参考文献/References:

[1]Kitano S,Shiraishi N,Kakisako K,et al.Laparoscopy assisted BillrothI gastrectomy (LADG) for cancer:our 10 years’ experience.Surg Laparosc Endosc Percutan Tech,2002,12:204-207.
[2]余佩武，王自强，钱锋，等.腹腔镜辅助胃癌根治术105例.中华外科杂志，2006，44:1303-1306.
[3]Ziqiang W,Feng Q,Zhimin C,et al.Comparison of laparoscopically assisted and open radical distal gastrectomy with extended lymphadenectomy for gastric cancer management.Surg Laparosc Endosc,2006,20:1738-1743.
[4]Kitano S,Iso Y,Moriyama M,et al.Laparoscopicassisted Billroth Ⅰ gastrectomy.Surg Laparosc Endosc,1994,4:146-148.
[5]Pugliese R,Maggioni D,Sansonna F,et al.Total and subtotal laparoscopic gastrectomy for adenocarcinoma.Surg Endosc,2007,21:21-27.
[6]Takiguchi S,Matsuura N,Hamada Y,et al.Influence of CO2 pneumoperitoneum during laparoscopic surgery on cancer cell growth.Surg Endosc,2000,14:41-44.
[7]Leng J,Lang J,Jiang Y,et al.Impact of different pressures and exposure times of a simulated carbon dioxide pneumoperitoneum environment on proliferation and apoptosis of human ovarian cancer cell lines.Surg Endosc,2006,20:1556-1559.
[8]徐春阳，梁志清.二氧化碳人工气腹对子宫内膜癌细胞体外生长的影响.中华妇产科杂志,2003，38：766-767.
[9]Gutt CN,Kim ZG,Hollander D,et al.CO2 environment influences the growth of cultured human cancer cells dependent on insufflation pressure.Endosc Surg,2001,15:314-318.
[10]Wildbrett P,Oh A,Naundorf D,et al.Impact of laparoscopic gases on peritoneal microenvironment and essential parameters of cell function.Surg Endosc,2003,17:78-82.

备注/Memo

备注/Memo:
基金项目：全军“十一五”计划课题资助项目（06MB240）；重庆市医学重点学科建设资助项目,**通讯作者①超声诊断科,
更新日期/Last Update: 2013-10-22