[1]张兵,夏春*,陈福①.大鼠腓肠肌内蛋白激酶B各亚型表达的年龄差异[J].中国微创外科杂志,2008,08(3):273-275.
　Zhang Bing*,Xia Chun,Chen Fu..Expression of Protein Kinase B Isoforms in the Gastrocnemius Muscle of Rats at Different Ages[J].Chinese Journal of Minimally Invasive Surgery,2008,08(3):273-275.
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大鼠腓肠肌内蛋白激酶B各亚型表达的年龄差异()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
08
期数:
2008年3期
页码:
273-275
栏目:
基础研究
出版日期:
2008-10-25

文章信息/Info

Title:
Expression of Protein Kinase B Isoforms in the Gastrocnemius Muscle of Rats at Different Ages
作者:
张兵; 夏春*; 陈福①
厦门大学医学院组织胚胎学教研室，厦门361005
Author(s):
Zhang Bing*;  Xia Chun;  Chen Fu.
*Department of Histology and Embryology, Medical College of Xiamen University, Xiamen 361005, China
关键词:
Akt1; Akt2; Akt3; 大鼠; 腓肠肌; 萎缩
Keywords:
Akt1; Akt2; Akt3; Rats; Gastrocnemius muscle; Atrophy
分类号:
R-332
文献标志码:
A
摘要:
目的研究大鼠腓肠肌内蛋白激酶B（Akt/PKB）各亚型伴随年龄变化的表达差异。方法用反转录PCR（RTPCR)、免疫印迹（western blotting）检测老年组（30月龄）和青年组(6月龄)大鼠腓肠肌内Akt各亚型mRNA和蛋白表达，并统计分析比较。结果（1）30月龄组大鼠腓肠肌内Akt1与Akt2的mRNA水平均高于6月龄组（t=7.124，P=0.000；t=2.598，P=0.021），Akt3差异无显著性（t=0.460，P=0.653）。（2）两组间Akt1蛋白表达水平无显著变化（t=0.469，P=0646）,30月龄组Akt1的Thr308磷酸化水平低于6月龄组的表达，有显著差异（t=-9.861，P=0.000）；30月龄组Akt2的蛋白水平高于6月龄组的表达，有显著差异（t=7.522，P=0.000）；Akt3蛋白表达在两组间无差异（t=0.058，P=0.955）。结论大鼠腓肠肌中Akt三种亚型伴随年龄改变表达存在差异，推测Akt各亚型在此过程中可能发挥不同功效。
Abstract:
ObjectiveTo study the expression of protein kinase B (Akt/PKB) isoforms in the gastrocnemius muscle of rats at different ages.MethodsThe expression levels of mRNA and protein of three Akt isoforms in the gastrocnemius muscle of 30monthold and 6monthold rats were detected respectively by RTPCR and Western blotting. Data were analyzed statistically.Results(1) The levels of Akt1 and Akt2 mRNA in the 30monthold rats was significantly higher than those in the 6monthold rats (t=7.124, P=0.000; t=2.598, P=0.021), however, no significant difference was found in the level of Akt3 mRNA between the two groups (t=0.460, P=0.653) . (2) The level of AktThr308 phosphorylation in the 30monthold rats was significantly lower than that in the 6monthold rats (t=-9.861, P=0.000), while the level of Akt2 protein in the 30month group was significantly higher than that in the younger rats (t=7.522, P=0.000). No significant differences were detected in the levels of Akt1 and Akt3 proteins between the two groups (t=0.469, P=0.646; t=0.058, P=0.955). ConclusionThe expression levels of three Akt isoforms in the gastrocnemius muscle of rats change with age, suggesting that the three isoforms of Akt have different functions in the gastrocnemius muscle metabolism of rats at different ages.

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备注/Memo

备注/Memo:
基金项目：福建省自然科学基金计划资助项目(C0310001)*通讯作者（厦门大学附属中山医院骨科，厦门361004）①（厦门大学医学院抗癌研究中心，厦门361005）
更新日期/Last Update: 2013-10-22