[1]丁士刚,吴笛①,邹检平②,等.食管鳞癌RASSF1A基因启动子区甲基化研究[J].中国微创外科杂志,2007,07(12):1213-1216.
　Ding Shigang*,Wu Di,Zou Jianping,et al.Hypermethylation Status of RASSF1A Gene Promoter Region in Esophageal Squamous Cell Carcinoma[J].Chinese Journal of Minimally Invasive Surgery,2007,07(12):1213-1216.
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食管鳞癌RASSF1A基因启动子区甲基化研究()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
07
期数:
2007年12期
页码:
1213-1216
栏目:
基础研究
出版日期:
2007-12-20

文章信息/Info

Title:
Hypermethylation Status of RASSF1A Gene Promoter Region in Esophageal Squamous Cell Carcinoma
作者:
丁士刚; 吴笛①; 邹检平②; 邢秀娟③; 鲁凤民③; 陈香梅**
北京大学第三医院消化科，北京100083
Author(s):
Ding Shigang*;  Wu Di; Zou Jianping; et al.
*Department of Digestive Diseases, Peking University Third Hospital, Beijing 100083, China
关键词:
食管鳞癌; 甲基化; RASSF1A; β-微管蛋白; 抑癌基因
Keywords:
Esophageal carcinoma; Methylation; RASSF1A; βtubulin; Tumor suppressor gene
分类号:
R735.1;R-33
文献标志码:
A
摘要:
目的研究肿瘤抑制基因RASSF1A启动子区甲基化所致该基因表达抑制在我国食管鳞癌发生中的作用及可能机制。方法用甲基化特异PCR技术（MSP）检测43例原发性食管鳞癌标本及6例对照食管上皮组织标本、食管鳞癌细胞系TE11和TE12中RASSF1A启动子甲基化状态；逆转录(RT)-PCR法检测5-氮-2′-脱氧胞苷(5AzaCdR)处理前后食管鳞癌细胞系中RASSF1A mRNA表达水平；Western blot方法检测食管鳞癌细胞系中细胞微管蛋白的表达。结果209%（9/43）原发性食管鳞癌标本有RA
Abstract:
ObjectiveTo study the involvement of promoter region hypermethylation induced gene silencing of tumor suppressor gene RASSF1A in the tumorigenesis of esophageal squamous cell carcinoma (ESCC).MethodsMethylation specific PCR (MSP) was employed to test the

参考文献/References:

[1]李卫东，王亮，王秀琴，等.食管癌组织染色体位点特异性的杂合性丢失和微卫星DNA序列不稳定.中华医学遗传学杂志,1996,13(4):194-197.
[2]An JY,Fan ZM,Wang LD,et al.Loss of heterozygosity in multistage carcinogenesis of esophageal carcinoma at highincidence area in Henan Province,China.World J Gastroenterol,2005,11(14):2055-2060.
[3]Wong ML,Tao Q,Fu L,et al.Aberrant promoter hypermethylation and silencing of the critical 3p21 tumour suppressor gene,RASSF1A,in Chinese oesophageal squamous cell carcinoma.Int J Oncol,2006,28(3):767-773.
[4]Kuroki T,Tamotsu S,Trapasso S,et al.Promoter hypermethylation of RASSF1A in esophageal squamous cell carcinoma.Clin Cancer Res,2003,9(4):1441-1445.
[5]Herman JG,Graff JR,Myohanen S,et al.Methylationspecific PCR: a novel PCR assay for methylation status of CpG islands.Proc Natl Acad Sci USA,1996,93(18):9821-9826.
[6]Gao H,Wang LD,Zhou Q,et al.p53 tumor suppressor gene mutation in early esophageal precancerous lesions and carcinoma among highrisk populations in Henan,China.Cancer Res,1994,54(16):4342-4346.
[7]Benzeno S,Lu F,Guo M,et al.Identification of mutations that disrupt phosphorylationdependent nuclear export of cyclin D1.Oncogene,2006,25(47):6291-6303.
[8]Guo M,Liu S,Lu F.Gefitinibsensitizing mutations in esophageal carcinoma.N Engl J Med,2006,354(20):2193-2194.
[9]Liu Z,Zhang L,Ding F,et al.5Aza2’deoxycytidine induces retinoic acid receptorbeta(2) demethylation and growth inhibition in esophageal squamous carcinoma cells.Cancer Lett,2005,230(2):271-283.
[10]Yamaguchi S,Kato H,Miyazaki T,et al.RASSF1A gene promoter methylation in esophageal cancer specimens.Dis Esophagus,2005,18(4):253-256.
[11]Kuroki T,Yendamuri S,Yendamuri S,et al.Allele loss and promoter hypermethylation of VHL,RAR beta,RASSF1A, and FHIT tumor suppressor genes on chromosome 3p in esophageal squamous cell carcinoma.Cancer Res,2003,63(13):3724-3728.
[12]杜季梅,李晓明,郭明洲,等.食管鳞癌雌激素受体α基因启动子甲基化异常.国际遗传学杂志,2006,29(6):401-403.
[13]Vos MD,Martinez AE,Candice DA,et al.A role for the RASSF1A tumor suppressor in the regulation of tubulin polymerization and genomic stability.Cancer Res,2004,64(12):4244-4250.

备注/Memo

备注/Memo:
基金项目:国家自然科学基金主任基金(No.30540081),**通讯作者(北京大学医学部病原生物学系，北京100083),①(广州市红十字会医院中医科，广州510220),②(沈阳药科大学神经病理学系，沈阳110016,)③(北京大学医学部病原生物学系，北京 100083)
更新日期/Last Update: 2013-12-10