[1]袁喜红,印慨,高军①,等.腹腔镜手术CO2气腹对绿色荧光蛋白标记活细胞血路肝迁徙的影响[J].中国微创外科杂志,2007,07(11):1108-1110.
　Yuan Xihong*,Yin Kai*,Gao Jun,et al.Effect of CO2 Pneumoperitoneum during Laparoscopy on Migration of GFPlabeled Living Cells into the Liver through Blood Route[J].Chinese Journal of Minimally Invasive Surgery,2007,07(11):1108-1110.
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腹腔镜手术CO2气腹对绿色荧光蛋白标记活细胞血路肝迁徙的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
07
期数:
2007年11期
页码:
1108-1110
栏目:
实验研究
出版日期:
2007-11-20

文章信息/Info

Title:
Effect of CO2 Pneumoperitoneum during Laparoscopy on Migration of GFPlabeled Living Cells into the Liver through Blood Route
作者:
袁喜红; 印慨; 高军①; 吴红玉①; 郑成竹
第二军医大学附属长海医院微创外科中心，上海200433
Author(s):
Yuan Xihong*;  Yin Kai*;  Gao Jun;  et al.
*Center of Microinvasive Surgery, Changhai Hospital, Second Military Medical University, Shanghai 200433, China
关键词:
人工气腹; 动物模型; 绿色荧光蛋白; 大鼠
Keywords:
Pneumoperitoneum;  artifical; Animal model; Green fluorescent protein; Rat
分类号:
R-332
文献标志码:
A
摘要:
目的探讨腹腔镜手术CO2气腹对绿色荧光蛋白（green fluorescent protein,GFP）标记活细胞血路肝迁徙的影响。方法将SD大鼠剖腹，经门静脉注入转染GFP的C57BL/6小鼠肝细胞（5×106cells/只），立即关腹后随机分成4组（每组10只），快速建立大鼠体内CO2气腹模型,采用持续性不同强度的CO2气腹压力（0、5、10、15 mm Hg）进行处理。30 min后处死动物取出肝脏，肝组织取样行快速冰冻切片检查，比较各组动物肝内荧光细胞的表达情况。结果各组GFP荧光细胞表达阳性率分别为：无气腹组8只，5 mm Hg气腹组9只，10 mm Hg气腹组9只和15 mm Hg气腹组10只，4组差异无统计学意义（χ2=2.222,P=0528）。各组GFP荧光细胞数目分别为：无气腹组6.63±2.45、5 mm Hg组7.67±2.83、10 mm Hg组13.89±4.37和15 mm Hg组15.50±6.29，4组GFP荧光细胞数目有显著统计学差异（F=10.78，P=0.000）；其中10 mm Hg和15 mm Hg气腹组GFP荧光细胞数目均明显高于无气腹气腹组和5 mm Hg气腹组，有显著统计学差异(P<0.05)。结论 腹腔镜手术CO2气腹能促进GFP标记活细胞经血路肝脏迁徙、积聚的发生，并且随着气腹压力的升高作用越明显。
Abstract:
ObjectiveTo study the effect of CO2 pneumoperitoneum on migration of GFPlabeled living cells into the liver through blood route in rat model. Methods SD rats was inoculated intraportally with highdose (5×106) GFPlabeled liver cells from C57BL/6 mice after cutting belly open. Pneumoperitoneum was established immediately after closing the abdominal wall. The rats were randomly divided into four groups (n=10 in each) to receive CO2 pneumoperitoneum at 5, 10, or 15 mmHg, or no treatment other than cells inoculation (control). The pneumoperitoneum was maintained for 30 min. Afterwards, the rats were euthanized by cervical dislocation, and the liver of the rats was removed for fast frozen section biopsy. The expression of GFPlabeled living cells in rat livers was compared between the groups. ResultsNo significant difference was detected in the positive expression of GFPlabeled cells between the groups (8 rats in the control group, 9 in the 5 mm Hg group, 9 in the 10 mm Hg group, and 10 in the 15 mm Hg group, χ2=2.222, P=0.528). The mean number of GFPpositive cells in the four groups was 6.63±2.45 (control), 7.67±283 (5 mm Hg), 13.89±4.37 (10 mm Hg), and 15.50±6.29 (15 mm Hg). There was significant differences between the four groups (F=10.78, P=0.000). In addition, the numbers of GFPpositive cells in the high pressure groups (15 mm Hg and 10 mm Hg) were significantly higher than that in the low pressure group (5 mm Hg) and the control (P<0.05).ConclusionsCO2 pneumoperitoneum can enhance the migration of GFPlabeled living cells into the liver through blood route. The effect of pneumoperitoneum increases with the pressure.

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备注/Memo

备注/Memo:
全军“十一五”面上项目，课题编号：06MA162.①（第二军医大学附属长海医院全军消化内科研究所，上海200433）
更新日期/Last Update: 2013-12-09