[1]许方婧伟,李潮*.明胶降解物对大鼠真皮成纤维细胞增殖及I型胶原蛋白分泌的影响[J].中国微创外科杂志,2007,07(8):815-817.
　Xufang Jingwei,Li Chao..The Influence of Gelatin Hydrolysate on Proliferation of Rat Dermis Fibroblasts and Type I Collagen Secretion[J].Chinese Journal of Minimally Invasive Surgery,2007,07(8):815-817.
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明胶降解物对大鼠真皮成纤维细胞增殖及I型胶原蛋白分泌的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
07
期数:
2007年8期
页码:
815-817
栏目:
实验研究
出版日期:
2007-08-20

文章信息/Info

Title:
The Influence of Gelatin Hydrolysate on Proliferation of Rat Dermis Fibroblasts and Type I Collagen Secretion
作者:
许方婧伟; 李潮*
北京大学第三医院心外科，北京100083
Author(s):
Xufang Jingwei;  Li Chao.
Department of Cardiovascular Surgery, Peking University Third Hospital, Beijing 100083, China
关键词:
明胶降解物; 真皮成纤维细胞; 细胞增殖; 胶原分泌
Keywords:
Gelatin hydrolysate; Dermis fibroblast; Cell proliferation; Collagen secretion
分类号:
R-332
文献标志码:
A
摘要:
目的探讨组织工程心瓣膜常用人工基质材料——明胶发生降解后的产物对大鼠真皮成纤维细胞增殖及I型胶原蛋白分泌的影响。方法利用酶组合技术制备明胶降解物（gelatin hydrolysate, GH），采用超滤的方法分离出低分子量明胶降解物组分（gelatin hydrolysate fraction, GHF），用于5只SD大鼠真皮成纤维细胞的体外培养。采用CCK-8活细胞计数试剂盒（Cell Counting Kit-8）检测细胞的增殖情况，ELISA试剂盒检测细胞I型胶原蛋白的分泌情况。 结果①GHF对细胞增殖作用的浓度效应：在0.125~10 mg/ml浓度范围内，GHF对细胞的促增殖作用呈现先增强后减弱的趋势，在0.25 mg/ml时，细胞增殖率最大，达到（47.54±16.35）%；②GH与GHF对细胞增殖影响比较：GH与GHF均具有促进细胞增殖的作用，且在浓度为0.25 mg/ml时，促细胞增殖作用无显著差异［(27.04±15.21)% vs (39.22±15.55)%, P=0.177］；③GH与GHF对细胞分泌I型胶原蛋白影响比较：GHF组第3天时I型胶原分泌量为（28.06±5.60）pg/105，与空白对照的（18.24±452）pg/105相比，有显著差异（P=0.006），而GH组直至第6天时I型胶原蛋白分泌量，与空白对照相比，无统计学意义（P=0.103）。结论分子量大小不同的明胶降解物GH与GHF均具有促进大鼠真皮成纤维细胞增殖的作用，且分子量较小的GHF还具有促进细胞I型胶原分泌的功能。
Abstract:
ObjectiveTo investigate the influences of hydrolysate of gelatin, a commonly used artificial material for cardiac valve surgeries, on rat dermis fibroblasts proliferation and type I collagen secretion.MethodsBioenzymatic hydrolysis technique was used for obtaining gelatin hydrolysate (GH), and ultra filtration for collecting the gelatin hydrolysate fractions (GHF) with lower molecular weight, which were applied in vitro culture of dermis fibroblasts from 5 SD rats. Cell proliferation and type I collagen secretion were measured with Cell Counting Kit8 and ELISA kit, respectively.Results①When the concentration fell in the range of 0.125-1.0 mg/ml, GHF could stimulate cell proliferation, especially at 0.25 mg/ml in which the proliferation rate reached the peak of (47.54±16.35)%; ②Compared with GHF, GH could also stimulate cell proliferation, and no significant difference was observed between them ［(27.04±15.21)% vs (39.22±15.55)%, P=0.177］ while the concentration was 0.25 mg/ml, even if GH had a higher molecular weight than GHF; ③The amount of type I collagen secreted by rat dermis fibroblasts cultured in GHF group on the 3rd day was up to (28.06±5.60)pg/105, significantly higher than the blank control (P=0.006). However, no significant increase in type I collagen secretion in fibroblasts cultured with GH was observed compared with the blank control group until the 6th day (P>0.05).ConclusionsBoth GH and GHF, gelatin hydrolysates with various molecular weights, can stimulate rat dermis fibroblasts proliferation, and the latter can meanwhile increase type I collagen secretion.

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备注/Memo

备注/Memo:
*通讯作者
更新日期/Last Update: 2013-12-09