[1]王艳滨①,陈敏华,严昆,等.原发性肝癌射频治疗后局部免疫功能的变化及其临床意义[J].中国微创外科杂志,2006,06(10):803-806.
　Wang Yanbin,Chen Minhua*,Yan Kun*,et al.Effects on local immunological functions after radiofrequency ablation for primary hepatocellular carcinoma[J].Chinese Journal of Minimally Invasive Surgery,2006,06(10):803-806.
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原发性肝癌射频治疗后局部免疫功能的变化及其临床意义()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
06
期数:
2006年10期
页码:
803-806
栏目:
实验研究
出版日期:
2006-10-30

文章信息/Info

Title:
Effects on local immunological functions after radiofrequency ablation for primary hepatocellular carcinoma
作者:
王艳滨①; 陈敏华; 严昆; 张;  霍苓
北京大学临床肿瘤学院,北京肿瘤医院超声诊断科,北京,100036
Author(s):
Wang Yanbin; Chen Minhua*;  Yan Kun*;  et al.
*Department of Ultrasonography, School of Oncology of Peking University, Beijing 100036, China
关键词:
原发性肝癌; 射频消融; 免疫组化; 热休克蛋白70; 肿瘤浸润淋巴细胞
Keywords:
Primary hepatocellular carcinoma; 　Radiofrequency ablation; 　Immunohistochemistry; 　Heat shock protein 70; 　Tumor infiltrating lymphocytes
分类号:
R735.7;R730.56;R446.6
文献标志码:
A
摘要:
目的 对原发性肝癌(hepatocellular carcinoma,HCC)射频消融治疗(radiofrequency ablation,RFA)前后肿瘤内部及边缘热休克蛋白70(heat shock protein,HSP70)的表达、CD8+T细胞数量的变化以及RFA治疗后,肿瘤边缘HSP70表达与肿瘤边缘CD8+T细胞数量之间的关系进行观察,探讨RFA治疗对原发性肝癌局部免疫功能状态的影响及其可能的临床意义.方法 对17例HCC分别在RFA治疗前、后1个月,于肿瘤内部和肿瘤边缘超声引导下穿刺活检取样;采用PowerVisionTM二步染色法进行免疫组化分析,测定HSP70的表达、CD8+T细胞的数量;随访HCC复发/新生情况.结果 RFA治疗后肿瘤边缘组织HSP70表达增强(Z=3.337,P=0.001)、CD8+T细胞数量增多(Z=1.996,P=0.049);RFA治疗后,≤4 cm肿瘤组的占位边缘CD8+T细胞数量高于＞4 cm肿瘤组(Z=1.966,P=0.048).RFA治疗后,边缘组织HSP70表达与CD8+T细胞数量之间呈正相关关系(r=0.489,P=0.046);RFA治疗后,无复发或新生组的占位边缘组织HSP70表达和CD8+T细胞数量分别高于复发或新生组(Z=2.009,P=0.045;Z=2.007,P=0.045). 结论 RFA治疗后边缘HSP70表达增强、CD8+T细胞数量增多,显示RFA治疗后局部免疫原性提高,抗肿瘤效应细胞浸润增加. 关键词：原发性肝癌;射频消融;免疫组化;热休克蛋白70;肿瘤浸润淋巴细胞
Abstract:
Objective　To investigate effects on local immunological functions and their clinical significances after radiofrequency ablation (RFA) for primary hepatocellular carcinoma (HCC).　M ethods　A total of 17 cases of primaryHCC was included in the study. Ultrasound-guided puncture and biopsywas carried outbefore and afterRFA for tumor sampling inside and at the border of theRFA sphere, respectively. The expression ofheatshock protein 70 (HSP70) and the numberofCD8+T cell in situwere analyzed with immunohistochemistry using the PowerVision Two-Step Detection System. Follow-up examinations were conducted for detecting tumor relapse or recurrence.　Results　Following the RFA, significantly up-regulated expression ofHSP70 (Z=3.337, P=0. 001) and increased numberofCD8+T cells (Z=1.996, P=0. 049) were seen at the border zone ofRFA sphere. The number ofCD8+T cellswas significantly higher in tumors≤4 cm in diameter than those > 4 cm in diameter (Z=1·966, P=0. 048). A positive relation was seen between HSP70 expression and CD8+T cell number at the border zone ofRFA sphere (r=0.489, P= 0·046). TheHSP70 expression and the CD8+T cellnumber at the border zone ofRFA spherewere higher in recurrence-free patients than in recurrentpatients, respectively (Z=2.009, P=0.045; Z=2.007, P=0. 045).　Conclusions　Following the RFA, the HSP70 expression and the CD8+T cell number at the border zone ofRFA sphere will be elevated, indicating thatRFA induces an immune response against tumor antigens and a tumor-specific T cell reaction.

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备注/Memo

备注/Memo:
基金项目：首都医学发展科研基金重点学科基金资助(基金编号 ZD 1999909)北京大学医学部"十五""211工程"重点学科建设项目经费资助(基金编号 523)，①（现在华北煤炭医学院附属医院超声科,唐山,063000）
更新日期/Last Update: 2014-03-03