[1]李宽新,史晨辉,王永明,等.金属蛋白酶-1和组织金属蛋白酶抑制因子-1在膝关节病理性滑膜皱襞中的表达及意义[J].中国微创外科杂志,2006,06(8):621-623.
　Li Kuanxin,Shi Chenhui,Wang Yongming,et al.Expression ofmatrix m etalloproteinase-1and tissue inhibitor ofm etalloproteinase-1 in pathologic synovial plicae of the knee joint[J].Chinese Journal of Minimally Invasive Surgery,2006,06(8):621-623.
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金属蛋白酶-1和组织金属蛋白酶抑制因子-1在膝关节病理性滑膜皱襞中的表达及意义()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
06
期数:
2006年8期
页码:
621-623
栏目:
实验研究
出版日期:
2006-08-20

文章信息/Info

Title:
Expression ofmatrix m etalloproteinase-1and tissue inhibitor ofm etalloproteinase-1 in pathologic synovial plicae of the knee joint
作者:
李宽新; 史晨辉; 王永明; 董金波; 刘维钢; 李茂强; 王维山
石河子大学医学院第一附属医院骨一科,石河子,832008
Author(s):
Li Kuanxin;  Shi Chenhui;  Wang Yongming;  et al.
Department of Orthopedics, First Affiliated Hospital of Medical College of Shihezi University, Shihezi 832008, China
关键词:
基质金属蛋白酶-1; 滑膜皱襞; 膝关节
Keywords:
Matrix metalloproteinase-1; 　Synovial plica; 　Knee joint
分类号:
R686.7;R446.6
文献标志码:
A
摘要:
目的 探讨病理性滑膜皱襞发病机制中对软骨破坏有基质金属蛋白酶的参与.方法 关节镜检查确诊为病理性滑膜皱襞和正常滑膜皱襞,分别进行免疫组化染色,观察金属蛋白酶-1(MMP-1)和组织金属蛋白酶抑制因子-1(TIMP-1)的表达及分布.结果 MMP-1、TIMP-1在病理性滑膜皱襞和正常皱襞内的阳性表达,差异具有显著性(χ2 ＝16.014,P=0.000;χ2 ＝4.059,P=0.044).MMP-1在滑膜衬里层细胞、单核和淋巴细胞、血管内皮细胞和化生的软骨细胞呈阳性表达,而在正常滑膜皱襞组织中不表达.TIMP-1只在滑膜衬里层细胞和少量的成纤维细胞有表达,而MMP-1免疫组化显示阳性细胞数和着色强度强于TIMP-1.结论 病理性滑膜皱襞可产生MMP-1、TIMP-1,而且两者分布不平衡,可能是导致软骨破坏的生物学因素.
Abstract:
Objective　To explorewhetherornot the cartilage damage in the pathogenesis ofpathologic synovialplicae is due to thematrixmetalloproteinase.　M ethods　The immunohistochemicalmethod was used to detect the expression and distribution of matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in arthroscopically clarified pathologic synovial plicae and normal synovial plicae of the knee joint.　Results　The positive expression rate ofMMP-1 and TIMP-1 had significantdifferences between the pathologic and normal synovial plicae (χ2=16.014, P=0. 000;χ2=4.059, P=0. 044). The expression ofMMP-1 was positive in the synovial lining cells, monocytes, lymphocytes, endothelial cells, and chondrocytes, but negative in the normal synovial plicae. The TIMP-1 expression was only detected in the synovial lining cells and a small quantity of fibroblast cells. The immunohistochemical analysis revealed a greater number of positive cells and intensity of staining ofMMP-1 than TIMP-1.　Conclusions　The development of pathologic synovial plicaemay yieldMMP-1 and TIMP-1 with unbalanced distributions, whichmay be the biological basis of the pathogenesis of cartilage destruction.

参考文献/References:

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[2]郭常安,陈峥嵘,张秀荣.骨关节炎基质金属蛋白酶1、2、3及其抑制物的表达与软骨退变的关系.复旦大学学报(医学板),2002,29(4):244-246.
[3]吴宏斌,杜靖远,郑启新,等.基质金属蛋白酶-1在创伤性骨关节炎软骨及滑膜中的表达.中华创伤杂志,2003,19(1):42-46.

更新日期/Last Update: 2014-02-13