[1]郭庆军①,裘正军,刘俊,等.CO2气腹对荷瘤大鼠IL-1β、IL-6和腹膜巨噬细胞功能的影响[J].中国微创外科杂志,2006,06(4):306-308.
　Guo Qingjun,Qiu Zhengjun*,Liu Jun*,et al.Effects of CO2pneumoperitoneum on levels of interleukin-1βand interleukin-6, and peritoneal macrophages in tumor- bearing rat[J].Chinese Journal of Minimally Invasive Surgery,2006,06(4):306-308.
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CO2气腹对荷瘤大鼠IL-1β、IL-6和腹膜巨噬细胞功能的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
06
期数:
2006年4期
页码:
306-308
栏目:
实验研究
出版日期:
2006-04-20

文章信息/Info

Title:
Effects of CO2pneumoperitoneum on levels of interleukin-1βand interleukin-6, and peritoneal macrophages in tumor- bearing rat
作者:
郭庆军①; 裘正军; 刘俊; 王瑞涛; 钟福全; 祝哲诚　
上海市第一人民医院普外科,上海,200080
Author(s):
Guo Qingjun;  Qiu Zhengjun*;  Liu Jun*;  et al.
Department of GeneralSurgery, Shanghai First People's Hospital,Shanghai 200080, China
关键词:
IL-1β; IL-6; 巨噬细胞; TNF-α
Keywords:
Interleukin-1β; 　Interleukin-6; 　Macrophage; 　Tumor necrosis factor-α
分类号:
R-332
文献标志码:
A
摘要:
目的采用种植性大鼠肝脏肿瘤模型观察CO2气腹对细胞因子和腹膜巨噬细胞功能的影响.方法荷瘤大鼠32只随机分为4组(n=8):对照组(仅麻醉)、开腹组、免气腹组和CO2气腹组.术后2、24 h收集血清,检测血清中的IL-1β、IL-6水平;术后48 h,收集培养腹膜巨噬细胞,检测巨噬细胞产生的TNF-α水平.结果术后2、24 h开腹组IL-6的水平分别为(57.92±2.06)pg/ml,(35.49±1.15)pg/ml,显著高于CO2气腹组(14.64±0.34)pg/ml,(15.39±0.86)pg/ml,免气腹组(24.75±1.53)pg/ml,(17.10±0.97)pg/ml和对照组(17.75±1.60)pg/ml,(14.55±0.25)pg/ml(P＜0.05).术后2 h开腹组IL-1β的水平为(92.63±4.81)pg/ml,显著高于CO2气腹组(57.94±4.46)pg/ml、免气腹组(58.48±3.20)pg/ml和对照组(58.99±2.30)pg/ml(P＜0.05).CO2气腹组腹膜巨噬细胞产生的TNF-α水平为(35.69±3.54)pg/ml,显著低于免气腹组(68.87±4.08)pg/ml、开腹组(82.96±5.39)pg/ml和对照组(66.45±7.96)pg/ml(P＜0.05).结论腹腔镜术后机体应激反应较小,CO2气腹可能抑制腹膜巨噬细胞的功能.
Abstract:
Objective　To investigate effects of CO2pneumoperitoneum on cytokines and peritonealmacrophages in a rat modelwith implanted liver tumor.　M ethods　A totalof32W istar ratswith implanted liver tumorwere randomly divided into4 groups (n=8): ControlGroup (anesthesia only), LaparotomyGroup, GaslessGroup (gasless laparoscopy), and Pneumoperitoneum Group ( laparoscopy underCO2pneumoperitoneum). Serum sampleswere collected at the 2nd and 24th hours after the procedure respectively for the detection of levels of interleukin-1β(IL-1β) and interleukin-6 (IL-6). Samples of peritonealmacrophageswere collected and incubated for the detection of levels of tumor necrosis factor-α(TNF-α), a product ofmacrophages.　Results　At the 2nd and 24th hours after surgery, levels of serum IL-6 in the LaparotomyGroup (57. 92±2. 06 pg/ml and 35. 49±1. 15 pg/ml) were significantly greater than those in the Pneumoperitoneum Group (14. 64±0. 34 pg/ml and 15. 39±0. 86 pg/ml), the Gasless Group (24. 75± 1·53 pg/ml and 17. 10±0. 97 pg/ml), and the ControlGroup (17. 75±1. 60 pg/ml and 14. 55±0. 25 pg/ml) (P<0. 05). Levels of serum IL-1βat the 2nd postoperative hourswere significantly higher in the Laparotomy Group (92. 63±4. 81 pg/ml) than in the Pneumoperitoneum Group (57. 94±4. 46 pg/ml), the GaslessGroup (58. 48±3. 20 pg/ml), and the ControlGroup (58. 99±2. 30 pg/ml) (P<0. 05). Levels ofTNF-αfrom peritonealmacrophageswere significantly lower in the Pneumoperitoneum Group (35. 69± 3. 54 pg/ml) than in the Gasless Group (68. 87±4. 08 pg/ml), the Laparotomy Group (82. 96±5. 39 pg/ml), and the Control Group (66. 45±7. 96 pg/ml) (P<0. 05).　Conclusions　During laparoscopic surgery the systemic stress reaction is notdistinctive. The carbon dioxide pneumoperitoneum may suppress functions of peritonealmacrophages.

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备注/Memo

备注/Memo:
①（天津市第一中心医院移植外科,天津,300192）
更新日期/Last Update: 2014-01-27