[1]曹月敏,宁殿宾,张万星,等.胆囊结石与胆囊收缩素受体(CCK-A)和血管活性肠肽(VIP)的关系研究[J].中国微创外科杂志,2005,05(10):868-870.
　Cao Yuemin,NingDianbin,ZhangWanxing,et al.On the relationship between gallstones and cholecystokinin-A receptor and vasoactive intestinal poly-peptide[J].Chinese Journal of Minimally Invasive Surgery,2005,05(10):868-870.
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胆囊结石与胆囊收缩素受体(CCK-A)和血管活性肠肽(VIP)的关系研究()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
05
期数:
2005年10期
页码:
868-870
栏目:
基础研究
出版日期:
2005-10-30

文章信息/Info

Title:
On the relationship between gallstones and cholecystokinin-A receptor and vasoactive intestinal poly-peptide
作者:
曹月敏; 宁殿宾; 张万星; 谭文科; 彭彦辉; 郭怀斌; 邢颖
河北省人民医院肝胆外科,石家庄,050051
Author(s):
Cao Yuemin;  NingDianbin;  ZhangWanxing;  et al
Department ofHepatobiliary Surgery, HebeiPeople’sHospital, Shijiazhuang050051, China
关键词:
胆囊收缩素受体; 血管活性肠肽; 放射免疫分析; 基因表达
Keywords:
Cholecystokinin receptor; 　Vasoactive intestinal poly-peptide; 　Radioimmunoassay; 　Gene expression
分类号:
R657.4
文献标志码:
A
摘要:
目的探讨胆囊动力学异常在胆囊结石形成中的作用.方法随意选择胆囊结石患者35例,胆囊息肉样病变患者25例,正常对照30例.B超测空腹胆囊容积.放射免疫法测定血浆中血管活性肠肽(VIP)的浓度,逆转录-聚合酶链反应(rt-PCR)法测胆囊黏膜中胆囊收缩素(CCK-A)受体表达数目.结果①胆囊结石组胆囊空腹体积明显大于其它两组(F=3.45,P=0.039);②胆囊结石组胆囊收缩率明显低于其它两组 (F=5.747,P=0.005);③三组之间餐后VIP升高量无明显差异 (F=0.768,P=0.47);④胆囊结石组胆囊收缩素(CCK-A)受体表达明显低于胆囊息肉样病变组(t′=4.390,P=0.022),差异具有显著性.结论①胆囊结石组空腹胆囊体积增大与其胆囊结石形成有关; ②胆囊结石组胆囊收缩功能下降导致结石形成;③胆囊收缩素(CCK-A)受体表达低,使胆囊收缩功能减弱,促进胆囊结石的形成;④血管活性肠肽(VIP)与胆囊结石的形成无明显关系.
Abstract:
Objective　To discuss the effect of abnormal biliary dynamics on the formation of cholelithiasis.　M ethods　A total of35 cases of gallstone (Gallstone Group), 25 cases of gallbladder polypoid lesions (Polypus Group), and 30 normal cases (ControlGroup) were included in this study. The fasting gallbladder volume was measured with B -ultrasonography, the serum concentration of vasoactive intestinal poly - peptide (VIP) was determined with radioimmunoassay, and the expression of cholecystokinin-A (CCK-A) receptor in the gallbladdermucousmembranewas detectedwith reverse transcriptase polymerase chain reactions (rt-PCR).　Results　①The fasting gallbladder volumewas significantly greater in the Gallstone Group than in the other two groups (F=3. 45,P=0. 039).②The gallbladder contraction ratewas significantly lower in theGallstoneGroup than in the other two groups (F=5. 747,P=0. 005).③The postprandial increases ofVIP of the three groupswere notof statistical significance (F= 0. 768,P=0. 47).④Compared with the PolypusGroup, the expression ofCCK-A receptor in theGallstoneGroupwas significantly decreased (tˊ=4. 390,P=0. 022).　Conclusions　①The increase of the fasting gallbladder volume in the Gallstone Group is associated with the formation of gallstones.②In the Gallstone Group, depressed gallbladder contraction results in the formation of gallstones.③The decreased expression ofCCK-A receptor leads to depressed gallbladder contraction, which facilitates the formation of gallstones.④Serum VIP is not related to the formation of gallstones.

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备注/Memo

备注/Memo:
基金项目：河北省自然基金资助项目(项目编号:302605)
更新日期/Last Update: 2014-04-29