[1]程树群,吴孟超,钱德初,等.超声消融术对癌细胞超微结构和细胞周期的影响[J].中国微创外科杂志,2005,05(2):119-121.
　ChengShuqun,WuMengchao,Qian Dechu,et al.Effect of ultrasound-mediated disruption on the ultrastructure and the cell cycle of hepatoma[J].Chinese Journal of Minimally Invasive Surgery,2005,05(2):119-121.
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超声消融术对癌细胞超微结构和细胞周期的影响()
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《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
05
期数:
2005年2期
页码:
119-121
栏目:
基础研究
出版日期:
2005-02-28

文章信息/Info

Title:
Effect of ultrasound-mediated disruption on the ultrastructure and the cell cycle of hepatoma
作者:
程树群; 吴孟超; 钱德初; 曹惠芳; 崔贞福
程树群，吴孟超，钱德初，曹惠芳，崔贞福
Author(s):
ChengShuqun;  WuMengchao; Qian Dechu;  et al.
Department of Surgery, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China
关键词:
超声消融术;  肝癌;  门静脉癌栓
Keywords:
Ultrasound-mediated disruption; 　Hepatic carcinoma; 　Tumor embolism of the portal vein
分类号:
R735.705.4
文献标志码:
A
摘要:
目的探讨超声消融术对癌细胞超微结构和细胞周期的影响. 方法体外超声消融(42 kHz,0.22 W)Walker-256细胞,扫描电镜、透射电镜和流式细胞仪分别观察细胞变化. 结果超声消融Walker-256细胞后,电镜观察发现:细胞膜破裂、细胞器紊乱不清、核染色质碎裂,提示细胞已受到严重的损伤.流式细胞仪检查发现,随着超声剂量的增大,细胞S期下降,G2+M期增加,说明细胞合成抑制,放射敏感性增加. 结论在细胞水平验证了超声消融术对癌细胞具有杀伤效应.
Abstract:
Objective　To observe changes of the ultrastructure and the cell cycle ofhepatoma cells exposed to ultrasound- mediated disruption.　M ethods　Suspensions ofWalker-256 hepatoma in vitro exposed to ultrasound disruption with the acoustic power output 0·22 W and the ultrasound frequency 42 kHz were observed by the scanning electron microscopy, the transmission electron microscopy and the flow cytometric analysis ofDNA content.　Results　Cells afterultrasound disruption revealed by scanning electronmicroscopy exhibited rupturedmembrance, disordered organellae and disrupted nucleus, showing typical severe destruction of the cells. Flow cytometric analysis ofDNA contentdemonstrated an increased in the population of cells in theG2andM phases of the cell cycle but a decrease in number of cells in the S phase, suggesting an inhibition of the cell synthese and an increased sensitivity to radiation.　Conclusions　Ultrasound disruption can destroy tumor cells effectively at the cellular leve.l

参考文献/References:

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更新日期/Last Update: 2014-03-11