[1]侯纯升①  Linda Wykes  John Hoffer.无创尿硫测定含硫氨基酸的分解代谢[J].中国微创外科杂志,2003,03(4):356-358.
 Hou Chunsheng,Linda Wykes,JohnHof- fer..Noninvasive detection of urinary sulfur for measuring the catabolism of sulfur amino acid[J].Chinese Journal of Minimally Invasive Surgery,2003,03(4):356-358.
点击复制

无创尿硫测定含硫氨基酸的分解代谢()
分享到:

《中国微创外科杂志》[ISSN:1009-6604/CN:11-4526/R]

卷:
03
期数:
2003年4期
页码:
356-358
栏目:
基础研究
出版日期:
2003-04-30

文章信息/Info

Title:
Noninvasive detection of urinary sulfur for measuring the catabolism of sulfur amino acid
作者:
侯纯升①  Linda Wykes  John Hoffer 
School of Dietetics and Human Nutrition, McGill University, Montreal, Quebec, Canada H9X 3V9
Author(s):
Hou ChunshengLinda WykesJohnHof- fer.
School ofDietetics andHuman Nutrition,McGill University,Montreal,CanadaH9X3V9
关键词:
含硫氨基酸 蛋氨酸 分解代谢 小猪
Keywords:
Sulfur amino acid Methionine Sulfur Catabolism Piglet
分类号:
R-332
文献标志码:
A
摘要:
目的验证尿硫测定是否可以在生长迅速的新出生的小猪模型上精确反映含硫氨基酸的分解代谢情况. 方法在有充分营养支持的小猪肠内营养模型上,观测静脉注射的无机硫酸盐、以及蛋氨酸中的硫是否完全经尿排泄. 结果以总硫方式测定的无机硫酸盐和蛋氨酸在尿中的回收率分别为的95.6%和105.5%. 结论在生长迅速的小猪模型上,尿硫测定这一无同位素标记、无创的方法可以精确反映含硫氨基酸的分解代谢情况.
Abstract:
Objective To test whether urinary sulfur excretion can be used as an accurate indicator of the catabolism of sulfur amino acid in growing newborn piglets. Methods Using a well-nourished enteral nutrition piglet model,we tested whether intravenous inorganic sulfate and methionine were fully excreted as sulfur in the urine. Results Recovery rate of inorganic sulfate and methionine as total sulfur in urine were 95·6% and 105·5%, respectively. Conclusions Detection of urinary sulfur, as a non-tracer and noninvasive method, may be employed to accurately measure the catabolism of sulfur amino acid in the growing piglet model.

参考文献/References:

[1]Hamadeh MJ,Hoffer LJ. Tracer methods underestimate short-term variations in urea production in humans. Am J Physiol,1998,274:E547-E553.
[2]Zello GA, Wykes LJ, Ball RO, et al. Recent advances in methods of assessing dietary amino acid requirements for adult humans. J Nutr,1995,125:2907-2915.
[3]Hoffer LJ. Evaluation of the adaptation to protein restriction in humans. In:El-Khoury AE,eds. Methods for the investigation of amino acid and protein metabolism.Boca Raton:CRC Press,1999.83-102.
[4]Bauer JH. Oral administration of radioactive sulfate to measure extracellular fluid space in man. J Appl Physiol,1976,40: 648-650.
[5]Sturman JA, Hepner GW, Hofmann AF,et al. Metabolism of [35s]taurine in man. J Nutr, 1975,105:1206-1214.
[6]Wykes LJ, Ball RO, Pencharz PB. Development and validation of a total parenteral nutrition model in the neonatal piglet. J Nutr,1993,123:1248-1259.
[7]Lundquist P, Martensson J, Sorbo B,et al. Turbidimetry of inorganic sulfate, ester sulfate, and total sulfur in urine. Clin Chem,1980,26:1178-1181.
[8]Robitaille L,Hoffer LJ. Measurement of branched chain amino acids in blood plasma by high-performance liquid chromatography. Can J Physiol Pharmacol,1988,66:613-617.
[9]Hum S, Robitaille L,Hoffer LJ. Plasma glutathione turnover in the rat: effect of fasting and buthionine sulfoximine. Can J Physiol Pharmacol,1991,69:581-587.
[10]Stipanuk MH. Homocysteine, cysteine, and taurine. In: Shils ME, Olson JA, Shike M,et al,eds.Modern Nutrition in Health and Disease.Baltimore: Williams & Wilkins, 1999.543-558.

备注/Memo

备注/Memo:
①(北京大学第三医院普外科,北京,100083)
更新日期/Last Update: 2014-07-02